Data from: Epigenetic mapping of the Arabidopsis metabolome reveals mediators of the epigenotype-phenotype map

  • Rik Kooke (Contributor)
  • Lionel Monteiro Morgado (Contributor)
  • Frank Becker (Contributor)
  • Henriëtte van Eekelen (Contributor)
  • Rashmi Hazarika (Contributor)
  • Qunfeng Zheng (Contributor)
  • Ric C.H. de Vos (Contributor)
  • Frank Johannes (Contributor)
  • Joost J. B. Keurentjes (Contributor)



    Identifying the sources of natural variation underlying metabolic differences between plants will enable a better understanding of plant metabolism and provide insights into the regulatory networks that govern plant growth and morphology. So far, however, the contribution of epigenetic variation to metabolic diversity has been largely ignored. In the present study, we utilized a panel of Arabidopsis thaliana epigenetic recombinant inbred lines (epiRILs) to assess the impact of epigenetic variation on the metabolic composition. Thirty epigenetic QTL (QTLepi) were detected, which partly overlap with QTLepi linked to growth and morphology. In an effort to identify causal candidate genes in the QTLepi regions or their putative trans-targets we performed in silico small RNA and qPCR analyses. Differentially expressed genes were further studied by phenotypic and metabolic analyses of knockout mutants. Three genes were detected that recapitulated the detected QTLepi effects, providing evidence for epigenetic regulation in cis and in trans. These results indicate that epigenetic mechanisms impact metabolic diversity, possibly via small RNAs, and thus aid in further disentangling the complex epigenotype-phenotype map.,EpiRILsand parentsQTL-mapping_rawLCMScdfsEpiRILs and parents QTL-mapping raw LCMS dataEpiRILs and parents QTL-mapping_raw LCMS cdfs.zipMutantsSupplTable7_rawLCMscdfsMutants Suppl Table 7_raw LCMs cdfs.zipsamplecodes_rawLCMScdfssample codes _raw LCMS cdfs.xlsx,
    Date made available4-Dec-2018
    PublisherUniversity of Groningen

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