Data from: Intestinal Ralstonia pickettii augments glucose intolerance in obesity

  • Shanthadevi Udayappan (Acad Med Ctr, Dept Internal & Vasc Med) (Creator)
  • Petia Kovatcheva-Datchary (Creator)
  • Guido J Bakker (Creator)
  • Stefan R. Havik (Creator)
  • Hilde Herrema (Creator)
  • Patrice D. Cani (Creator)
  • Kristien E Bouter (Creator)
  • Clara Belzer (Creator)
  • Julia J. Witjes (Creator)
  • Anne Vrieze (Creator)
  • Noor de Sonnaville (Creator)
  • Alice Chaplin-Scott (Creator)
  • Daniël H van Raalte (Creator)
  • Steven Aalvink (Creator)
  • Geesje Dallinga-Thie (Creator)
  • Hans G. H. J. Heilig (Creator)
  • Goran Bergstrom (Creator)
  • Suzan van der Meij (Creator)
  • Bart A. van Wagensveld (Creator)
  • Joost B. L. Hoekstra (Creator)
  • Frits Holleman (Creator)
  • Erik S. G. Stroes (Creator)
  • Bert Groen (Creator)
  • Fredrik Backhed (Creator)
  • Willem M. de Vos (Creator)
  • Max Nieuwdorp (Creator)



An altered intestinal microbiota composition has been implicated in the pathogenesis of metabolic disease including obesity and type 2 diabetes mellitus (T2DM). Low grade inflammation, potentially initiated by the intestinal microbiota, has been suggested to be a driving force in the development of insulin resistance in obesity. Here, we report that bacterial DNA is present in mesenteric adipose tissue of obese but otherwise healthy human subjects. Pyrosequencing of bacterial 16S rRNA genes revealed that DNA from the Gram-negative species Ralstonia was most prevalent. Interestingly, fecal abundance of Ralstonia pickettii was increased in obese subjects with pre-diabetes and T2DM. To assess if R. pickettii was causally involved in development of obesity and T2DM, we performed a proof-of-concept study in diet-induced obese (DIO) mice. Compared to vehicle-treated control mice, R. pickettii-treated DIO mice had reduced glucose tolerance. In addition, circulating levels of endotoxin were increased in R. pickettii-treated mice. In conclusion, this study suggests that intestinal Ralstonia is increased in obese human subjects with T2DM and reciprocally worsens glucose tolerance in DIO mice.
Date made available1-Dec-2017
PublisherUniversity of Groningen

Keywords on Datasets

  • microbiome
  • obesity
  • glucose intolerance
  • inflammation
  • Ralstonia pickettii
  • Intestinal Ralstonia pickettii augments glucose intolerance in obesity

    Udayappan, S. D., Kovatcheva-Datchary, P., Bakker, G. J., Havik, S. R., Herrema, H., Cani, P. D., Bouter, K. E., Belzer, C., Witjes, J. J., Vrieze, A., de Sonnaville, N., Chaplin, A., van Raalte, D. H., Aalvink, S., Dallinga-Thie, G. M., Heilig, H. G. H. J., Bergstrom, G., van der Meij, S., van Wagensveld, B. A., Hoekstra, J. B. L., & 6 othersHolleman, F., Stroes, E. S. G., Groen, A. K., Backhed, F., de Vos, W. M. & Nieuwdorp, M., 22-Nov-2017, In: PLoS ONE. 12, 11, 15 p., e0181693.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
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