Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching

Dataset

Description

This dataset contains all data and scripts used to generate figure 1, "Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching". - The ImageJ macro ("Macro_bleaching.ijm") is used to background correct the unprocessed bleaching movies, which are stored in the folders "Bleaching GFP (movies)" and "Bleaching RFP (movies)" respectively. - The Python notebook ("Fig1_data_analysis.ipynb") contains all code needed to analyze the bleaching movies and generate figure 1.
Date made available21-Feb-2022
PublisherDataverseNL

Keywords on Datasets

  • Budding yeast
  • Saccharomyces cerevisiae
  • START
  • cell cycle commitment
  • Whi5
  • Cln3
  • microscopy

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