Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching

  • Athanasios Litsios (Contributor)
  • Pooja Goswami (Contributor)
  • Hanna Terpstra (Contributor)
  • Carleton Coffin (Contributor)
  • Luc-Alban Pierre Eric Vuillemenot (Contributor)
  • Mattia Rovetta (Contributor)
  • Ghada Ghazal (Contributor)
  • Paolo Guerra (Contributor)
  • Katarzyna Buczak (Contributor)
  • Alexander Schmidt (Contributor)
  • Sylvain Tollis (Contributor)
  • Mike Tyers (Contributor)
  • Catherine A. Royer (Contributor)
  • Andreas Milias Argeitis (Contributor)
  • Matthias Heinemann (Contributor)

Dataset

Description

This dataset contains all data and scripts used to generate figure 1, "Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching". - The ImageJ macro ("Macro_bleaching.ijm") is used to background correct the unprocessed bleaching movies, which are stored in the folders "Bleaching GFP (movies)" and "Bleaching RFP (movies)" respectively. - The Python notebook ("Fig1_data_analysis.ipynb") contains all code needed to analyze the bleaching movies and generate figure 1.
Date made available21-Feb-2022
PublisherDataverseNL

Keywords on Datasets

  • Budding yeast
  • Saccharomyces cerevisiae
  • START
  • cell cycle commitment
  • Whi5
  • Cln3
  • microscopy

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