Dataset Figure_2: Determination of nuclear Whi5 concentration by widefield fluorescence microscopy is confounded by non-proportional scaling of the nuclear-to-cell volume

Athnasios Litsios (Contributor)
Pooja Goswami (Contributor)
Hanna Terpstra (Contributor)
Carleton Coffin (Contributor)
Luc-Alban Pierre Eric Vuillemenot (Contributor)
Mattia Rovetta (Contributor)
Ghada Ghazal (Contributor)
Paolo Guerra (Contributor)
Katarzyna Buczak (Contributor)
Alexander Schmidt (Contributor)
Sylvain Tollis (Contributor)
Mike Tyers (Contributor)
Catherine A. Royer (Contributor)
Andreas Milias Argeitis (Contributor)
Matthias Heinemann (Contributor)

Molecular Systems Biology

Dataset

Description

This dataset contain the data used to determine the nuclear to whole-cell volume for the Nup133-yeGFP strain.

Date made available	24-Feb-2022
Publisher	DataverseNL

Keywords on Datasets

Budding yeast
Saccharomyces cerevisiae
START
cell cycle commitment
Whi5
Cln3
microscopy

Contact

[email protected]

DOI
10.34894/tfc012

The timing of Start is determined primarily by increased synthesis of the Cln3 activator rather than dilution of the Whi5 inhibitor
Litsios, A., Goswami, P., Terpstra, H. M., Coffin, C., Vuillemenot, L.-A., Rovetta, M., Ghazal, G., Guerra, P., Buczak, K., Schmidt, A., Tollis, S., Tyers, M., Royer, C. A., Milias-Argeitis, A. & Heinemann, M., 1-May-2022, In: Molecular Biology of the Cell. 33, 5, 14 p., rp2.
Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

Open Access
File

12 Citations (Scopus)

268 Downloads (Pure)

Dataset Figure_6: Cln3 levels pulse prior to Start
Litsios, A. (Contributor), Goswami, P. (Contributor), Terpstra, H. (Contributor), Coffin, C. (Contributor), Vuillemenot, L.-A. P. E. (Contributor), Rovetta, M. (Contributor), Ghazal, G. (Contributor), Guerra, P. (Contributor), Buczak, K. (Contributor), Schmidt, A. (Contributor), Tollis, S. (Contributor), Tyers, M. (Contributor), Royer, C. A. (Contributor), Milias-Argeitis, A. (Contributor) & Heinemann, M. (Contributor), DataverseNL, 21-Feb-2022
DOI: 10.34894/pkzzoj
Dataset
Dataset Figure_5: Whi5-GFP intensity versus size and time in a synchronous G1 population
Litsios, A. (Contributor), Goswami, P. (Contributor), Terpstra, H. (Contributor), Coffin, C. (Contributor), Vuillemenot, L.-A. P. E. (Contributor), Rovetta, M. (Contributor), Ghazal, G. (Contributor), Guerra, P. (Contributor), Buczak, K. (Contributor), Schmidt, A. (Contributor), Tollis, S. (Contributor), Tyers, M. (Contributor), Royer, C. A. (Contributor), Milias-Argeitis, A. (Contributor) & Heinemann, M. (Contributor), DataverseNL, 21-Feb-2022
DOI: 10.34894/hnwbym
Dataset
Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching
Litsios, A. (Contributor), Goswami, P. (Contributor), Terpstra, H. (Contributor), Coffin, C. (Contributor), Vuillemenot, L.-A. P. E. (Contributor), Rovetta, M. (Contributor), Ghazal, G. (Contributor), Guerra, P. (Contributor), Buczak, K. (Contributor), Schmidt, A. (Contributor), Tollis, S. (Contributor), Tyers, M. (Contributor), Royer, C. A. (Contributor), Milias-Argeitis, A. (Contributor) & Heinemann, M. (Contributor), DataverseNL, 21-Feb-2022
DOI: 10.34894/swrbyr
Dataset

Cite this

DataSetCite

Litsios, A. (Contributor), Goswami, P. (Contributor), Terpstra, H. (Contributor), Coffin, C. (Contributor), Vuillemenot, L. P. E. (Contributor), Rovetta, M. (Contributor), Ghazal, G. (Contributor), Guerra, P. (Contributor), Buczak, K. (Contributor), Schmidt, A. (Contributor), Tollis, S. (Contributor), Tyers, M. (Contributor), Royer, C. A. (Contributor), Milias-Argeitis, A. (Contributor), Heinemann, M. (Contributor) (24-Feb-2022). Dataset Figure_2: Determination of nuclear Whi5 concentration by widefield fluorescence microscopy is confounded by non-proportional scaling of the nuclear-to-cell volume. DataverseNL. 10.34894/tfc012

Dataset Figure_2: Determination of nuclear Whi5 concentration by widefield fluorescence microscopy is confounded by non-proportional scaling of the nuclear-to-cell volume

Description

Keywords on Datasets

Contact

DOI

Research output

The timing of Start is determined primarily by increased synthesis of the Cln3 activator rather than dilution of the Whi5 inhibitor

Datasets

Dataset Figure_6: Cln3 levels pulse prior to Start

Dataset Figure_5: Whi5-GFP intensity versus size and time in a synchronous G1 population

Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching

Cite this