Dataset Figure_4: Nuclear Whi5-GFP intensity versus time from repeated imaging of the same individual cells

  • Athanasios Litsios (Contributor)
  • Pooja Goswami (Contributor)
  • Hanna Terpstra (Contributor)
  • Carleton Coffin (Contributor)
  • Luc-Alban Pierre Eric Vuillemenot (Contributor)
  • Mattia Rovetta (Contributor)
  • Ghada Ghazal (Contributor)
  • Paolo Guerra (Contributor)
  • Katarzyna Buczak (Contributor)
  • Alexander Schmidt (Contributor)
  • Sylvain Tollis (Contributor)
  • Mike Tyers (Contributor)
  • Catherine A. Royer (Contributor)
  • Andreas Milias Argeitis (Contributor)
  • Matthias Heinemann (Contributor)



Whi5-GFP intensity as a function of time for the same FOVs. Only small daughter cells in the asynchronous population at time point 0 were quantified as a function of time. The folder 'Image Files' contains raw image files for Whi5-GFP for FOV1,3,4,5 & 6 at each of the 6 time points (0, 20, 40, 60, 80, 100 min). The folder 'Excel_analysis_output' contains the output files for all five FOV (1,3,4,5,6) for 1. fovX_t0 or time 100_whi5_nadh_*.xlsx – at time 0 and time 100 minutes. These files correspond to the analysis of the background auto-fluorescence excited at 750 nm used to calculate size. The only relevant information from these analyses is the Cyto size (fL) column in tab 3 of each file 2. fovX_tX_whi5_*.xlsx Analysis of all time points and all FOV for Whi5-GFP intensities.
Date made available21-Feb-2022

Keywords on Datasets

  • Budding yeast
  • Saccharomyces cerevisiae
  • cell cycle commitment
  • Whi5
  • Cln3
  • microscopy

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