This dataset contains the raw data for the publication "Assessing motor-related phenotypes of Caenorhabditis elegans with the wide field-of-view nematode tracking platform". Here you can find the associated raw data from Figs. 3, 4, 5, 6, 7, 13 and 14 belonging to the Nature Protocols paper: ‘Assessing motor-related phenotypes of Caenorhabditis elegans with the wide field-of-view nematode tracking platform’. All data was generated with the WF-NTP software and not manipulated afterwards unless stated otherwise. For the figures the next data was used from each dataset: Figure 3: only the raw data for eccentricity and BPM (bends per minute) was used. Figure 4: only the raw data for BPM, average and maximum speed (mm/s) was used. Figure 5: only the raw data for maximum speed (mm/s) was used. Figure 6: this is a processed file, for both D1 and D8 old worms we compared the ‘detected’ number of worms per movie by the WF-NTP with the actual number of worms that was manually counted. Delta provides the difference. Also, the file contains several tabs in which the worm presence is annotated (How many frames was each worm present). The supplementary tables generated from this data are also included. Figure 7: only the raw data for average speed (mm/s) was used. Figure 13: only the raw data for BPM was used and compared with the manual counted data. Figure 14: only the raw data for BPM was used.