Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle

  • Vakil Takhaveev (ETH Zürich) (Creator)
  • Serdar Ozsezen (Creator)
  • Edward Smith (Creator)
  • Andre Zylstra (Creator)
  • Marten L. Chaillet (Creator)
  • Haoqi Chen (Creator)
  • Alexandros Papagiannakis (Stanford University) (Creator)
  • Andreas Milias Argeitis (Creator)
  • Matthias Heinemann (Creator)

Dataset

Description

The ZIP files contain analysis files (Python) for the figures, as well as files for the two used models, i.e. the cell mass model and the FBA model. The analysis is described in detail in the related publication. (2023) Specifically, the different ZIP files contain: Figure1.zip: - Data and script for analysis karyokinesis time; - Data and script for analysis protein synthesis rate with stop-and-respond method (replicate 1); - Data and script for analysis protein synthesis rate with stop-and-respond method (replicate 2); - Data and script for analysis protein synthesis rate with sfGFP. Figure2.zip: - Data and script for cell surface and cell volume dynamics; - Data and script for analysis lipid synthesis rate (replicate 1); - Data and script for analysis lipid synthesis rate (replicate 1); - Data and script for analysis polysaccharide synthesis rate (replicate 1); - Data and script for analysis polysaccharide synthesis rate (replicate 2); - Data and script for control analysis polysaccharide synthesis rate. Figure3.zip: - Cell mass model. Figure4.zip: - Cell cycle FBA model; - Data and script for experiment with glucose analog uptake (replicate 1); - Data and script for experiment with glucose analog uptake (replicate 2); - Data and script for glycolytic flux sensor experiment. Figure5.zip: - Data and script for analysis of NADPH oscillations; - Data and script for microaerobic conditions and storage mutants.
Date made available7-Jan-2023
PublisherDataverseNL

Cite this