Research output per year
Research output per year
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2024
Insights into the Structure–Function Relationship of GH70 GtfB α-Glucanotransferases from the Crystal Structure and Molecular Dynamic Simulation of a Newly Characterized Limosilactobacillus reuteri N1 GtfB Enzyme
Dong, J., Bai, Y., Wang, Q., Chen, Q., Li, X., Wang, Y., Ji, H., Meng, X., Pijning, T., Svensson, B., Dijkhuizen, L., Abou Hachem, M. & Jin, Z., 13-Mar-2024, In: Journal of Agricultural and Food Chemistry. 72, 10, p. 5391-5402 12 p., acs.jafc.4c00104.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile5 Citations (Scopus) -
Novel β-galactosidase activity and first crystal structure of Glycoside Hydrolase family 154
Hameleers, L., Pijning, T., Gray, B. B., Fauré, R. & Jurak, E., 25-May-2024, In: New Biotechnology. 80, p. 1-11 11 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile73 Downloads (Pure) -
Polysaccharide utilization loci encoded DUF1735 likely functions as membrane-bound spacer for carbohydrate active enzymes
Hameleers, L., Gaenssle, L. A., Bertran-Llorens, S., Pijning, T. & Jurak, E., Jul-2024, In: FEBS Open Bio. 14, 7, p. 1133-1146 14 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile14 Downloads (Pure) -
Structural and time-resolved mechanistic investigations of protein hydrolysis by the acidic proline-specific endoprotease from Aspergillus niger
Pijning, T., Vujičić-Žagar, A., Laan, J.-M. V. D., Jong, R. M. D., Ramirez-Palacios, C., Vente, A., Edens, L. & Dijkstra, B. W., Jan-2024, In: Protein Science. 33, 1, 21 p., e4856.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile1 Citation (Scopus)42 Downloads (Pure) -
Tailor-Made α-Glucans by Engineering the Processivity of α-Glucanotransferases via Tunnel-Cleft Active Center Interconversions
Dong, J., Abou Hachem, M., Wang, Y., Li, X., Zhang, B., Pijning, T., Svensson, B., Dijkhuizen, L., Jin, Z. & Bai, Y., 15-May-2024, In: Journal of Agricultural and Food Chemistry. 72, 19, p. 11041–11050 10 p.Research output: Contribution to journal › Article › Academic › peer-review
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Unprecedented Diversity of the Glycoside Hydrolase Family 70: A Comprehensive Analysis of Sequence, Structure, and Function
Pijning, T. & Dijkhuizen, L., 31-Jul-2024, In: Journal of Agricultural and Food Chemistry. 72, 30, p. 16911-16929 19 p., 4c04807.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile2 Downloads (Pure) -
2023
Enzymatic glucosylation of polyphenols using glucansucrases and branching sucrases of glycoside hydrolase family 70
Li, X., Meng, X., de Leeuw, T. C., Te Poele, E. M., Pijning, T., Dijkhuizen, L. & Liu, W., 2023, In: Critical Reviews in Food Science and Nutrition. 63, 21, p. 5247–5267 21 p., 2016598.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile4 Citations (Scopus)265 Downloads (Pure) -
2022
Characterization of the (Engineered) Branching Sucrase GtfZ-CD2 from Apilactobacillus kunkeei for Efficient Glucosylation of Benzenediol Compounds
Meng, X., Li, X., Pijning, T., Wang, X., van Leeuwen, S. S., Dijkhuizen, L., Chen, G. & Liu, W., 4-Aug-2022, In: Applied and environmental microbiology. 88, 16, 14 p., e0103122.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile2 Citations (Scopus)71 Downloads (Pure) -
Crystal Structure of 4,6-α-Glucanotransferase GtfC-ΔC from Thermophilic Geobacillus 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes
Pijning, T., Te Poele, E. M., de Leeuw, T. C., Guskov, A. & Dijkhuizen, L., 7-Dec-2022, In: Journal of Agricultural and Food Chemistry. 70, 48, p. 15283–15295 13 p., 2c06394.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile2 Citations (Scopus)60 Downloads (Pure) -
Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity
Xu, W., Ni, D., Hou, X., Pijning, T., Guskov, A., Rao, Y. & Mu, W., 27-Apr-2022, In: Journal of Agricultural and Food Chemistry. 70, 16, p. 5095-5105 11 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile9 Citations (Scopus)127 Downloads (Pure) -
Mutations in Amino Acid Residues of Limosilactobacillus reuteri 121 GtfB 4,6-α-Glucanotransferase that Affect Reaction and Product Specificity
Jiang, Y., Li, X., Pijning, T., Bai, Y. & Dijkhuizen, L., 16-Feb-2022, In: Journal of Agricultural and Food Chemistry. 70, 6, p. 1952-1961 10 p., 1c07618.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile6 Citations (Scopus)95 Downloads (Pure) -
2021
Crystal structure of an inulosucrase from Halalkalicoccus jeotgali B3T-a halophilic archaeal strain
Ghauri, K., Pijning, T., Munawar, N., Ali, H., Ghauri, M. A., Anwar, M. A. & Wallis, R., Oct-2021, In: The FEBS Journal. 288, 19, p. 5723-5736 14 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile8 Citations (Scopus)140 Downloads (Pure) -
Insights into Broad-Specificity Starch Modification from the Crystal Structure of Limosilactobacillus Reuteri NCC 2613 4,6-α-Glucanotransferase GtfB
Pijning, T., Gangoiti, J., Te Poele, E. M., Börner, T. & Dijkhuizen, L., 10-Nov-2021, In: Journal of Agricultural and Food Chemistry. 69, 44, p. 13235-13245 11 p., 05657.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile20 Citations (Scopus)101 Downloads (Pure) -
Using Mutability Landscapes To Guide Enzyme Thermostabilization
Guo, C., Ni, Y., Biewenga, L., Pijning, T., Thunnissen, A.-M. W. H. & Poelarends, G. J., 5-Jan-2021, In: ChemBioChem. 22, 1, p. 170-175 7 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile6 Citations (Scopus)148 Downloads (Pure) -
2019
Enantioselective Synthesis of Pharmaceutically Active γ-Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions
Biewenga, L., Thangavelu, S., Kunzendorf, A., Van Der Meer, J.-Y., Pijning, T., Tepper, P., Van Merkerk, R., Charnock, S. J., Thunnissen, A.-M. W. H. & Poelarends, G. J., 1-Feb-2019, In: ACS Catalysis. 9, p. 1503-1513 11 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile45 Citations (Scopus)186 Downloads (Pure) -
Identification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity
Zhang, X., Leemhuis, R., Janecek, S., Martinovicova, M., Pijning, T. & van der Maarel, M., Aug-2019, In: Applied Microbiology and Biotechnology. 103, 15, p. 6141–6151 11 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile12 Citations (Scopus)193 Downloads (Pure) -
2018
Biochemical and genetic characterization of fungal proline hydroxylase in echinocandin biosynthesis
Zhang, F., Liu, H., Zhang, T., Pijning, T., Yu, L., Zhang, W., Liu, W. & Meng, X., Sept-2018, In: Applied Microbiology and Biotechnology. 102, 18, p. 7877-7890 14 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile12 Citations (Scopus)160 Downloads (Pure) -
Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity
Meng, X., Gangoiti, J., Wang, X., Grijpstra, P., van Leeuwen, S. S., Pijning, T. & Dijkhuizen, L., Sept-2018, In: Applied Microbiology and Biotechnology. 102, 18, p. 7935-7950 16 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile23 Citations (Scopus)132 Downloads (Pure) -
Biochemical characterization of two GH70 family 4,6-α-glucanotransferases with distinct product specificity from Lactobacillus aviarius subsp. aviarius DSM 20655
Meng, X., Gangoiti, J., de Kok, N., van Leeuwen, S. S., Pijning, T. & Dijkhuizen, L., 1-Jul-2018, In: Food Chemistry. 253, p. 236-246 11 p., j.foodchem.2018.01.154.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile31 Citations (Scopus)389 Downloads (Pure) -
Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose
Gangoiti, J., Pijning, T. & Dijkhuizen, L., Feb-2018, In: Biotechnology Advances. 36, p. 196-207 12 p.Research output: Contribution to journal › Review article › peer-review
Open AccessFile76 Citations (Scopus)185 Downloads (Pure) -
Branched alpha glucans
Gangoiti Muñecas, J. (Inventor), van Leeuwen, S. S. (Inventor), Pijning, T. (Inventor), Dijkhuizen, L. (Inventor), Vafeiadi, C. (Inventor) & Duboux, S. (Inventor), 20-Sept-2018, Patent No. WO2018167032, Priority date 15-Mar-2017Research output: Patent
Open Access -
Mutational Analysis of the Role of the Glucansucrase Gtf180-Delta N Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate
Pham, H., Pijning, T., Dijkhuizen, L. & van Leeuwen, S. S., 28-Nov-2018, In: Journal of Agricultural and Food Chemistry. 66, 47, p. 12544-12554 11 p., acs.jafc.8b04486.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile7 Citations (Scopus)238 Downloads (Pure) -
2017
4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H
Gangoiti Muñecas, J., van Leeuwen, S. S., Gerwig, G. J., Duboux, S., Vafiadi, C., Pijning, T. & Dijkhuizen, L., 6-Jan-2017, In: Scientific Reports. 7, p. 1-15 15 p., 39761.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile48 Citations (Scopus)384 Downloads (Pure) -
A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy
Franken, L., Oostergetel, G., Pijning, T., Puri, P., Arkhipova, V. I., Boekema, E., Poolman, B. & Guskov, A., 28-Sept-2017, In: Nature Communications. 8, 11 p., 722.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile43 Citations (Scopus)434 Downloads (Pure) -
A new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad
Sarian, F. D., Janeček, Š., Pijning, T., Ihsanawati, Nurachman, Z., Radjasa, O. K., Dijkhuizen, L., Natalia, D. & van der Maarel, M. J. E. C., 2017, In: Scientific Reports. 7, 10 p., 44230.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile34 Citations (Scopus)349 Downloads (Pure) -
Biochemical characterization of the functional roles of residues in the active site of the β-galactosidase from Bacillus circulans ATCC 31382
Yin, H., Pijning, T., Meng, X., Dijkhuizen, L. & van Leeuwen, S. S., 2017, In: Biochemistry. 56, 24, p. 3109-3118 9 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile14 Citations (Scopus)398 Downloads (Pure) -
Characterization of the glucansucrase GTF180 W1065 mutant enzymes producing polysaccharides and oligosaccharides with altered linkage composition
Meng, X., Pijning, T., Tietema, M., Dobruchowska, J. M., Yin, H., Gerwig, G. J., Kralj, S. & Dijkhuizen, L., 15-Feb-2017, In: Food Chemistry. 217, p. 81-90 10 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile34 Citations (Scopus)116 Downloads (Pure) -
Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria
Bai, Y., Gangoiti , J., Dijkstra, B. W., Dijkhuizen, L. & Pijning, T., 7-Feb-2017, In: Structure. 25, 2, p. 231-242 12 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile55 Citations (Scopus)426 Downloads (Pure) -
Engineering of the Bacillus circulans β-galactosidase product specificity
Yin, H., Pijning, T., Meng, X., Dijkhuizen, L. & van Leeuwen, S. S., 16-Jan-2017, In: Biochemistry. 56, 5, p. 704-711 8 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile34 Citations (Scopus)234 Downloads (Pure) -
Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB
Gangoiti, J., van Leeuwen, S. S., Meng, X., Duboux, S., Vafiadi, C., Pijning, T. & Dijkhuizen, L., 30-Aug-2017, In: Scientific Reports. 7, 1, 16 p., 9947.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile34 Citations (Scopus)337 Downloads (Pure) -
2016
Crystal Structure of a 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria
Pijning, T., Bai, Y., Gangoiti Muñecas, J. & Dijkhuizen, L., 11-Sept-2016.Research output: Contribution to conference › Poster › Academic
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Lactobacillus reuteri strains convert starch and maltodextrins into homo-exopolysaccharides using an extracellular and cell-associated 4,6-α-glucanotransferase
Bai, Y., Böger, M., van der Kaaij, R. M., Woortman, A. J. J., Pijning, T., van Leeuwen, S. S., Lammerts van Bueren, A. & Dijkhuizen, L., 2016, In: Journal of Agricultural and Food Chemistry. 64, 14, p. 2941-2952 11 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile30 Citations (Scopus)325 Downloads (Pure) -
Structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of Lactobacillus reuteri
Meng, X., Pijning, T., Dobruchowska, J. M., Yin, H., Gerwig, G. J. & Dijkhuizen, L., 2016, In: Scientific Reports. 6, 14 p., 35261.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile17 Citations (Scopus)394 Downloads (Pure) -
Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes
Meng, X., Gangoiti, J., Bai, Y., Pijning, T., Van Leeuwen, S. S. & Dijkhuizen, L., Jul-2016, In: Cellular and molecular life sciences. 73, 14, p. 2681-2706 26 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile72 Citations (Scopus)392 Downloads (Pure) -
Synthesis of New Hyper-Branched α-Glucans from Sucrose by Lactobacillus reuteri 180 Glucansucrase Mutants
Meng, X., Dobruchowska, J. M., Pijning, T., Gerwig, G. J. & Dijkhuizen, L., 20-Jan-2016, In: Journal of Agricultural and Food Chemistry. 64, 2, p. 433-442 10 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile27 Citations (Scopus)80 Downloads (Pure) -
2015
Biochemical characterization of Lactobacillus reuteri Glycoside Hydrolase family 70 GTFB type of 4,6-α-Glucanotransferase enzymes that synthesize soluble dietary starch fibers
Bai, Y., van der Kaaij, R. M., Leemhuis, H., Pijning, T., van Leeuwen, S. S., Jin, Z. & Dijkhuizen, L., 7-Aug-2015, In: Applied and environmental microbiology. 81, 20, p. 7223-7232 10 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile63 Citations (Scopus)47 Downloads (Pure) -
Characterization of the functional roles of amino acid residues in acceptor binding subsite +1 in the active site of the glucansucrase GTF180 enzyme of Lactobacillus reuteri 180
Meng, X., Pijning, T., Dobruchowska, J. M., Gerwig, G. J. & Dijkhuizen, L., 11-Dec-2015, In: The Journal of Biological Chemistry. 290, 50, p. 30131-30141 11 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile34 Citations (Scopus)242 Downloads (Pure) -
The Exiguobacterium sibiricum 255-15 GtfC enzyme representing a novel glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes
Gangoiti, J., Pijning, T. & Dijkhuizen, L., 20-Nov-2015, In: Applied and environmental microbiology. 82, 2, p. 756-766 11 p., AEM.03420-15.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile44 Citations (Scopus)35 Downloads (Pure) -
Truncation of domain V of the multidomain glucansucrase GTF180 of Lactobacillus reuteri 180 heavily impairs its polysaccharide-synthesizing ability
Meng, X., Dobruchowska, J. M., Pijning, T., Gerwig, G. J., Kamerling, J. P. & Dijkhuizen, L., Jul-2015, In: Applied Microbiology and Biotechnology. 99, 14, p. 5885-5894 10 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile26 Citations (Scopus)71 Downloads (Pure) -
2014
Flexibility of truncated and full-length glucansucrase GTF180 enzymes from Lactobacillus reuteri 180
Pijning, T., Vujicic - Zagar, A., Kralj, S., Dijkhuizen, L. & Dijkstra, B. W., May-2014, In: Febs Journal. 281, 9, p. 2159-2171 13 p.Research output: Contribution to journal › Article › Academic › peer-review
21 Citations (Scopus) -
Improved activity and pH stability of E-coli ATCC 11105 penicillin acylase by error-prone PCR
Balci, H., Ozturk, M. T., Pijning, T., Ozturk, S. I. & Gumusel, F., May-2014, In: Applied Microbiology and Biotechnology. 98, 10, p. 4467-4477 11 p.Research output: Contribution to journal › Article › Academic › peer-review
19 Citations (Scopus) -
Just add sugar... Structural Studies on Glucansucrase and Fructansucrase Enzymes from Lactobacilli
Pijning, T., 10-Jan-2014, Groningen: s.n.. 152 p.Research output: Thesis › Thesis fully internal (DIV)
Open AccessFile3608 Downloads (Pure) -
Residue Leu(940) Has a Crucial Role in the Linkage and Reaction Specificity of the Glucansucrase GTF180 of the Probiotic Bacterium Lactobacillus reuteri 180
Meng, X., Dobruchowska, J. M., Pijning, T., Lopez-Bautista, C., Kamerling, J. P. & Dijkhuizen, L., 21-Nov-2014, In: The Journal of Biological Chemistry. 289, 47, p. 32773-32782 10 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile35 Citations (Scopus)237 Downloads (Pure) -
2013
4,6-alpha-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily
Leemhuis, H., Dijkman, W. P., Dobruchowska, J. M., Pijning, T., Grijpstra, P., Kralj, S., Kamerling, J. P., Dijkhuizen, L. & Gerwig, G. J., Jan-2013, In: Applied Microbiology and Biotechnology. 97, 1, p. 181-193 13 p.Research output: Contribution to journal › Article › Academic › peer-review
Open AccessFile61 Citations (Scopus)908 Downloads (Pure) -
Glucansucrases: Three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications
Leemhuis, H., Pijning, T., Dobruchowska, J. M., van Leeuwen, S. S., Kralj, S., Dijkstra, B. W. & Dijkhuizen, L., 20-Jan-2013, In: Journal of Biotechnology. 163, 2, p. 250-272 23 p.Research output: Contribution to journal › Article › Academic › peer-review
258 Citations (Scopus)424 Downloads (Pure) -
Improved activity and thermostability of Bacillus pumilus lipase by directed evolution
Akbulut, N., Ozturk, M. T., Pijning, T., Ozturk, S. I. & Gumusel, F., 10-Mar-2013, In: Journal of Biotechnology. 164, 1, p. 123-129 7 p.Research output: Contribution to journal › Article › Academic › peer-review
72 Citations (Scopus) -
2012
Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: Factors contributing to increased activity retention
Augustyniak, W., Brzezinska, A. A., Pijning, T., Wienk, H., Boelens, R., Dijkstra, B. W. & Reetz, M. T., Apr-2012, In: Protein Science. 21, 4, p. 487-497 11 p.Research output: Contribution to journal › Article › Academic › peer-review
50 Citations (Scopus)161 Downloads (Pure) -
Functional and Structural Characterization of alpha-(1 -> 2) Branching Sucrase Derived from DSR-E Glucansucrase
Brison, Y., Pijning, T., Malbert, Y., Fabre, E., Mourey, L., Morel, S., Potocki-Veronese, G., Monsan, P., Tranier, S., Remaud-Simeon, M. & Dijkstra, B. W., 9-Mar-2012, In: The Journal of Biological Chemistry. 287, 11, p. 7915-7924 10 p.Research output: Contribution to journal › Article › Academic › peer-review
77 Citations (Scopus)116 Downloads (Pure) -
Glycosidic bond specificity of glucansucrases: on the role of acceptor substrate binding residues
Leemhuis, H., Pijning, T., Dobruchowska, J. M., Dijkstra, B. W. & Dijkhuizen, L., May-2012, In: Biocatalysis and Biotransformation. 30, 3, p. 366-376 11 p.Research output: Contribution to journal › Article › Academic › peer-review
54 Citations (Scopus)198 Downloads (Pure) -
Structure of the alpha-1,6/alpha-1,4-specific glucansucrase GTFA from Lactobacillus reuteri 121
Pijning, T., Vujicic-Zagar, A., Kralj, S., Dijkhuizen, L. & Dijkstra, B. W., Dec-2012, In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 68, 12, p. 1448-1454 7 p.Research output: Contribution to journal › Article › Academic › peer-review
56 Citations (Scopus)104 Downloads (Pure)