14-weeks combined exercise epigenetically modulated 118 genes of menopausal women with prediabetes

Natália Yumi Noronha, Guilherme da Silva Rodrigues*, Isabella Harumi Yonehara Noma, Camila Fernanda Cunha Brandao, Karine Pereira Rodrigues, Alexandre Colello Bruno, Chanachai Sae-Lee, Lígia Moriguchi Watanabe, Marcela Augusta de Souza Pinhel, Isabelle Mello Schineider, Mariana Luciano de Almeida, Fernando Barbosa Júnior, Déborah Araújo Morais, Wellington Tavares de Sousa Júnior, Torsten Plösch, Carlos Roberto Bueno Junior, Carla Barbosa Nonino

*Corresponding author for this work

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Background: Pre-diabetes precedes Diabetes Mellitus (DM) disease and is a critical period for hyperglycemia treatment, especially for menopausal women, considering all metabolic alterations due to hormonal changes. Recently, the literature has demonstrated the role of physical exercise in epigenetic reprogramming to modulate the gene expression patterns of metabolic conditions, such as hyperglycemia, and prevent DM development. In the present study, we hypothesized that physical exercise training could modify the epigenetic patterns of women with poor glycemic control.

Methods: 48 post-menopause women aged 60.3 ± 4.5 years were divided according to their fasting blood glucose levels into two groups: Prediabetes Group, PG (n=24), and Normal Glucose Group, NGG (n=24). All participants performed 14 weeks of physical exercise three times a week. The Infinium Methylation EPIC BeadChip measured the participants’ Different Methylated Regions (DMRs).

Results: Before the intervention, the PG group had 12 DMRs compared to NGG. After the intervention, five DMRs remained different. Interestingly, when comparing the PG group before and after training, 118 DMRs were found. The enrichment analysis revealed that the genes were related to different biological functions such as energy metabolism, cell differentiation, and tumor suppression.

Conclusion: Physical exercise is a relevant alternative in treating hyperglycemia and preventing DM in post-menopause women with poor glycemic control.

Original languageEnglish
Article number895489
Number of pages15
JournalFrontiers in endocrinology
Publication statusPublished - 15-Aug-2022


  • combined physical exercise
  • DNA methylation
  • epigenomics
  • older women
  • physical exercise

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