Abstract
Blue pigmentation in Streptomyces coelicolor A3(2) is attributed to synthesis of the polyketide actinorhodin and its lactone derivative γ-actinorhodin, Therefore, actinorhodin-negative mutants show pigmentation other than blue. When the B22 mutant of S.coelicolor A3(2) [defective in the actVI-ORF1 gene coding for a putative keto( = oxo)reductase] was examined for its secondary metabolite content, the presence of aloesaponarin II (3,8-dihydroxy-1-methyl-9,10-anthraquinone) as the major pigment was confirmed. However, a substantial fraction of a red/blue (acid/alkaline) pigment was detected after separation on HPLC. MS and NMR analysis revealed its structure as 3,5,8-trihydroxy-1-methyl-9,10-anthraquinone. To our knowledge, this anthraquinone has not previously been reported in biological material. A possible route for biosynthesis of this compound is discussed.
Original language | English |
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Pages (from-to) | 195-201 |
Number of pages | 7 |
Journal | Biotechnology and applied biochemistry |
Volume | 26 |
DOIs | |
Publication status | Published - Dec-1997 |
Keywords
- BIOSYNTHETIC GENE-CLUSTER
- ANTIBIOTIC ACTINORHODIN
- ENGINEERED BIOSYNTHESIS
- HYBRID ANTIBIOTICS
- ANTHRAQUINONES
- POLYKETIDES
- DERIVATIVES
- SEQUENCE
- REGION