A CDK-independent metabolic oscillator orchestrates the budding yeast cell cycle

A. Papagiannakis, B. Niebel, E. Wit, M. Heinemann

Research output: Contribution to journalMeeting AbstractAcademic


Eukaryotic cell division is known to be controlled by the cyclin/ CDK machinery. However, eukaryotes have evolved prior to CDKs, and cells can divide in the absence of major cyclin/CDK components. We hypothesized that an autonomous metabolic oscillator provides dynamic triggers for cell cycle initiation and progression. Using microfluidics, cell cycle reporters, and singlecell metabolite measurements, we found that metabolism of budding yeast is a CDK-independent oscillator that oscillates across different growth conditions, both in synchrony with and also in the absence of the cell cycle. Using environmental perturbations and dynamic single-protein depletion experiments, we found that the metabolic oscillator and the cell cycle form a system of coupled oscillators, with the metabolic oscillator separately coordinating both the early and late cell cycle. Establishing metabolism as a dynamic cell cycle regulator opens new avenues for cell cycle research and therapeutic interventions for proliferative disorders.
Original languageEnglish
Article numberS.5.4-002
Pages (from-to)54
Number of pages1
JournalFebs Journal
Issue numberS1
Publication statusPublished - 8-Sept-2017
Event42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back - Jerusalem, Israel
Duration: 10-Sept-201714-Sept-2017


  • cyclin dependent kinase
  • endogenous compound
  • budding yeast
  • cell cycle
  • metabolite
  • microfluidics
  • nonhuman
  • oscillator
  • protein depletion
  • yeast cell


Dive into the research topics of 'A CDK-independent metabolic oscillator orchestrates the budding yeast cell cycle'. Together they form a unique fingerprint.

Cite this