Abstract
Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (TH2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a TH2-dominated interactome in asthmatic lungs.
Original language | English |
---|---|
Pages (from-to) | 1153-1163 |
Number of pages | 24 |
Journal | Nature Medicine |
Volume | 25 |
Issue number | 7 |
Early online date | 17-Jun-2019 |
DOIs | |
Publication status | Published - Jul-2019 |
Keywords
- EPITHELIAL-CELLS
- PROSTAGLANDIN D-2
- RNA-SEQ
- EXPRESSION
- INFLAMMATION
- PATHOGENESIS
- MACROPHAGES
- PHENOTYPE
- DISEASE
- GENE
Fingerprint
Dive into the research topics of 'A cellular census of human lungs identifies novel cell states in health and in asthma'. Together they form a unique fingerprint.Datasets
-
vieira19_Nasal_anonymised-processed.csv
Fasouli, E. S. (Contributor), Theis, F. J. (Contributor), Talavera-López, C. (Contributor), Kar, G. (Contributor), Vieira Braga, F. A. (Contributor), Gomes, T. (Contributor), Meyer, K. B. (Contributor), Saeb-Parsy, K. (Contributor), Simon, L. M. (Contributor), Affleck, K. (Contributor), Luinge, M. (Contributor), Brandsma, C.-A. (Contributor), Jonker, M. R. (Contributor), Jiang, J. (Contributor), Nawijn, M. C. (Contributor), van den Berge, M. (Contributor), Strunz, M. (Contributor), Brouwer, S. (Contributor), Vento-Tormo, R. (Contributor), Mahbubani, K. T. (Contributor), Angelidis, I. (Contributor), Efremova, M. (Contributor), Teichmann, S. A. (Contributor), Hesse, L. (Contributor), Timens, W. (Contributor), Palit, S. (Contributor), Berg, M. (Contributor), Schiller, H. B. (Contributor), van Oosterhout, A. J. (Contributor), Firth, H. V. (Contributor), Polanski, K. (Contributor), Koppelman, G. H. (Contributor), Cvejic, A. (Contributor), Carpaij, O. A. (Contributor) & Strzelecka, P. M. (Contributor), University of Groningen, 20-Aug-2020
DOI: 10.6084/m9.figshare.12831938.v1, https://doi.org/10.6084%2Fm9.figshare.12831938.v1
Dataset
Press/Media
-
-
UMCG maakt 'plattegrond' van luchtwegen, die kan helpen bij de strijd tegen astma
18/06/2019
1 item of Media coverage
Press/Media: Research › Popular
-