A circadian clock in Saccharomyces cerevisiae

Zheng Eelderink-Chen, Gabriella Mazzotta, Marcel Sturre, Jasper Bosman, Till Roenneberg, Martha Merrow*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)
292 Downloads (Pure)

Abstract

Circadian timing is a fundamental biological process, underlying cellular physiology in animals, plants, fungi, and cyanobacteria. Circadian clocks organize gene expression, metabolism, and behavior such that they occur at specific times of day. The biological clocks that orchestrate these daily changes confer a survival advantage and dominate daily behavior, for example, waking us in the morning and helping us to sleep at night. The molecular mechanism of circadian clocks has been sketched out in genetic model systems from prokaryotes to humans, revealing a combination of transcriptional and posttranscriptional pathways, but the clock mechanism is far from solved. Although Saccharomyces cerevisiae is among the most powerful genetic experimental systems and, as such, could greatly contribute to our understanding of cellular timing, it still remains absent from the repertoire of circadian model organisms. Here, we use continuous cultures of yeast, establishing conditions that reveal characteristic clock properties similar to those described in other species. Our results show that metabolism in yeast shows systematic circadian entrainment, responding to cycle length and zeitgeber (stimulus) strength, and a (heavily damped) free running rhythm. Furthermore, the clock is obvious in a standard, haploid, auxotrophic strain, opening the door for rapid progress into cellular clock mechanisms.

Original languageEnglish
Pages (from-to)2043-2047
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number5
DOIs
Publication statusPublished - 2-Feb-2010

Keywords

  • entrainment
  • oscillation
  • yeast
  • temperature cycle
  • GENE-EXPRESSION
  • NEUROSPORA-CRASSA
  • PLASMA-MEMBRANE
  • FEEDBACK LOOP
  • CELL-CYCLE
  • YEAST
  • OSCILLATIONS
  • ENTRAINMENT
  • RHYTHMS
  • TRANSCRIPTION

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