A Cleavable Linker Based on the Levulinoyl Ester for Activity-Based Protein Profiling

Paul P. Geurink, Bogdan I. Florea, Nan Li, Martin D. Witte, Joeri Verasdonck, Chi-Lin Kuo, Gijs A. van der Marel, Herman S. Overkleeft

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The title linker is stable under various biological conditions and can be cleaved chemoselectively with hydrazine. Its use is demonstrated in the activity-based enrichment and identification of proteasome active subunits from cell extracts.
Original languageEnglish
Pages (from-to)6802-6805
Number of pages4
JournalAngewandte Chemie International Edition
Issue number38
Publication statusPublished - 2010


  • proteomics
  • proteasomes
  • levulinoyl ester
  • cleavable linkers
  • activity-based profiling

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