A Cleavable Linker Based on the Levulinoyl Ester for Activity-Based Protein Profiling

Paul P. Geurink, Bogdan I. Florea, Nan Li, Martin D. Witte, Joeri Verasdonck, Chi-Lin Kuo, Gijs A. van der Marel, Herman S. Overkleeft

Research output: Contribution to journalArticleAcademicpeer-review

40 Citations (Scopus)
850 Downloads (Pure)

Abstract

The title linker is stable under various biological conditions and can be cleaved chemoselectively with hydrazine. Its use is demonstrated in the activity-based enrichment and identification of proteasome active subunits from cell extracts.
Original languageEnglish
Pages (from-to)6802-6805
Number of pages4
JournalAngewandte Chemie International Edition
Volume49
Issue number38
DOIs
Publication statusPublished - 2010

Keywords

  • proteomics
  • proteasomes
  • levulinoyl ester
  • cleavable linkers
  • activity-based profiling

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