A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2

R Morgan; MHW Hooiveld; PID Rieden; AJ Durston

A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2

R Morgan^*, MHW Hooiveld, PID Rieden, AJ Durston

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern observed for Wnt-5a at the late gastrula stage. Further we show that gastrula stage Wnt-5a expression is repressed by otx2, something which is reflected by the mutually exclusive expression patterns of these two genes. The isolated promoter sequence contains an OTX- consensus binding site and its' activity in embryos is repressed by ectopically expressed otx2. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	97-102
Number of pages	6
Journal	Mechanisms of development
Volume	85
Issue number	1-2
Publication status	Published - Jul-1999

Keywords

Wnt-5a promoter
Xenopus
LacZ gene
ORTHODENTICLE GENE
ECTOPIC EXPRESSION
HEAD DEVELOPMENT
XENOPUS-EMBRYOS
HOMEOBOX GENE
IDENTIFICATION
PROTEIN
CELLS
GASTRULATION
HOMOLOG

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title = "A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2",

abstract = "We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern observed for Wnt-5a at the late gastrula stage. Further we show that gastrula stage Wnt-5a expression is repressed by otx2, something which is reflected by the mutually exclusive expression patterns of these two genes. The isolated promoter sequence contains an OTX- consensus binding site and its' activity in embryos is repressed by ectopically expressed otx2. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.",

keywords = "Wnt-5a promoter, Xenopus, LacZ gene, ORTHODENTICLE GENE, ECTOPIC EXPRESSION, HEAD DEVELOPMENT, XENOPUS-EMBRYOS, HOMEOBOX GENE, IDENTIFICATION, PROTEIN, CELLS, GASTRULATION, HOMOLOG",

author = "R Morgan and MHW Hooiveld and PID Rieden and AJ Durston",

year = "1999",

month = jul,

language = "English",

volume = "85",

pages = "97--102",

journal = "Mechanisms of development",

issn = "0925-4773",

publisher = "ELSEVIER SCIENCE BV",

number = "1-2",

}

TY - JOUR

T1 - A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2

AU - Morgan, R

AU - Hooiveld, MHW

AU - Rieden, PID

AU - Durston, AJ

PY - 1999/7

Y1 - 1999/7

N2 - We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern observed for Wnt-5a at the late gastrula stage. Further we show that gastrula stage Wnt-5a expression is repressed by otx2, something which is reflected by the mutually exclusive expression patterns of these two genes. The isolated promoter sequence contains an OTX- consensus binding site and its' activity in embryos is repressed by ectopically expressed otx2. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

AB - We have characterised a short (30 base pair) element from the Xenopus Wnt-5a promoter which is nearly identical to one located in the human Wnt-5a promoter, and has the same position relative to the transcription start site. When placed in front of a LacZ gene, this element can reproduce the same expression pattern observed for Wnt-5a at the late gastrula stage. Further we show that gastrula stage Wnt-5a expression is repressed by otx2, something which is reflected by the mutually exclusive expression patterns of these two genes. The isolated promoter sequence contains an OTX- consensus binding site and its' activity in embryos is repressed by ectopically expressed otx2. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

KW - Wnt-5a promoter

KW - Xenopus

KW - LacZ gene

KW - ORTHODENTICLE GENE

KW - ECTOPIC EXPRESSION

KW - HEAD DEVELOPMENT

KW - XENOPUS-EMBRYOS

KW - HOMEOBOX GENE

KW - IDENTIFICATION

KW - PROTEIN

KW - CELLS

KW - GASTRULATION

KW - HOMOLOG

M3 - Article

SN - 0925-4773

VL - 85

SP - 97

EP - 102

JO - Mechanisms of development

JF - Mechanisms of development

IS - 1-2

ER -

A conserved 30 base pair element in the Wnt-5a promoter is sufficient both to drive its' early embryonic expression and to mediate its' repression by otx2

Abstract

Keywords

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