A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation

Yixian Cui; Smriti Parashar; Muhammad Zahoor; Patrick G Needham; Muriel Mari; Ming Zhu; Shuliang Chen; Hsuan-Chung Ho; Fulvio Reggiori; Hesso Farhan; Jeffrey L Brodsky; Susan Ferro-Novick

doi:10.1126/science.aau9263

A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation

Yixian Cui, Smriti Parashar, Muhammad Zahoor, Patrick G Needham, Muriel Mari, Ming Zhu, Shuliang Chen, Hsuan-Chung Ho, Fulvio Reggiori, Hesso Farhan, Jeffrey L Brodsky, Susan Ferro-Novick^*

^*Corresponding author for this work

Microbes in Health and Disease (MHD)

Research output: Contribution to journal › Article › Academic › peer-review

125 Citations (Scopus)

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Abstract

The COPII-cargo adaptor complex Lst1-Sec23 selectively sorts proteins into vesicles that bud from the endoplasmic reticulum (ER) and traffic to the Golgi. Improperly folded proteins are prevented from exiting the ER and are degraded. ER-phagy is an autophagic degradation pathway that uses ER-resident receptors. Working in yeast, we found an unexpected role for Lst1-Sec23 in ER-phagy that was independent from its function in secretion. Up-regulation of the stress-inducible ER-phagy receptor Atg40 induced the association of Lst1-Sec23 with Atg40 at distinct ER domains to package ER into autophagosomes. Lst1-mediated ER-phagy played a vital role in maintaining cellular homeostasis by preventing the accumulation of an aggregation-prone protein in the ER. Lst1 function appears to be conserved because its mammalian homolog, SEC24C, was also required for ER-phagy.

Original language	English
Pages (from-to)	53-60
Number of pages	8
Journal	Science
Volume	365
Issue number	6448
DOIs	https://doi.org/10.1126/science.aau9263
Publication status	Published - 5-Jul-2019

Keywords

UNFOLDED PROTEIN RESPONSE
SELECTIVE AUTOPHAGY
CARGO
YEAST
LUNAPARK
PATHWAYS
TURNOVER
TRIGGERS
REQUIRES
TEX264

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A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradationFinal publisher's version, 1.2 MBLicence: Taverne

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@article{afaca571e29d4234a672838dea8f4bba,

title = "A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation",

abstract = "The COPII-cargo adaptor complex Lst1-Sec23 selectively sorts proteins into vesicles that bud from the endoplasmic reticulum (ER) and traffic to the Golgi. Improperly folded proteins are prevented from exiting the ER and are degraded. ER-phagy is an autophagic degradation pathway that uses ER-resident receptors. Working in yeast, we found an unexpected role for Lst1-Sec23 in ER-phagy that was independent from its function in secretion. Up-regulation of the stress-inducible ER-phagy receptor Atg40 induced the association of Lst1-Sec23 with Atg40 at distinct ER domains to package ER into autophagosomes. Lst1-mediated ER-phagy played a vital role in maintaining cellular homeostasis by preventing the accumulation of an aggregation-prone protein in the ER. Lst1 function appears to be conserved because its mammalian homolog, SEC24C, was also required for ER-phagy.",

keywords = "UNFOLDED PROTEIN RESPONSE, SELECTIVE AUTOPHAGY, CARGO, YEAST, LUNAPARK, PATHWAYS, TURNOVER, TRIGGERS, REQUIRES, TEX264",

author = "Yixian Cui and Smriti Parashar and Muhammad Zahoor and Needham, {Patrick G} and Muriel Mari and Ming Zhu and Shuliang Chen and Hsuan-Chung Ho and Fulvio Reggiori and Hesso Farhan and Brodsky, {Jeffrey L} and Susan Ferro-Novick",

note = "Copyright {\textcopyright} 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.",

year = "2019",

month = jul,

day = "5",

doi = "10.1126/science.aau9263",

language = "English",

volume = "365",

pages = "53--60",

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T1 - A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation

AU - Cui, Yixian

AU - Parashar, Smriti

AU - Zahoor, Muhammad

AU - Needham, Patrick G

AU - Mari, Muriel

AU - Zhu, Ming

AU - Chen, Shuliang

AU - Ho, Hsuan-Chung

AU - Reggiori, Fulvio

AU - Farhan, Hesso

AU - Brodsky, Jeffrey L

AU - Ferro-Novick, Susan

PY - 2019/7/5

Y1 - 2019/7/5

N2 - The COPII-cargo adaptor complex Lst1-Sec23 selectively sorts proteins into vesicles that bud from the endoplasmic reticulum (ER) and traffic to the Golgi. Improperly folded proteins are prevented from exiting the ER and are degraded. ER-phagy is an autophagic degradation pathway that uses ER-resident receptors. Working in yeast, we found an unexpected role for Lst1-Sec23 in ER-phagy that was independent from its function in secretion. Up-regulation of the stress-inducible ER-phagy receptor Atg40 induced the association of Lst1-Sec23 with Atg40 at distinct ER domains to package ER into autophagosomes. Lst1-mediated ER-phagy played a vital role in maintaining cellular homeostasis by preventing the accumulation of an aggregation-prone protein in the ER. Lst1 function appears to be conserved because its mammalian homolog, SEC24C, was also required for ER-phagy.

AB - The COPII-cargo adaptor complex Lst1-Sec23 selectively sorts proteins into vesicles that bud from the endoplasmic reticulum (ER) and traffic to the Golgi. Improperly folded proteins are prevented from exiting the ER and are degraded. ER-phagy is an autophagic degradation pathway that uses ER-resident receptors. Working in yeast, we found an unexpected role for Lst1-Sec23 in ER-phagy that was independent from its function in secretion. Up-regulation of the stress-inducible ER-phagy receptor Atg40 induced the association of Lst1-Sec23 with Atg40 at distinct ER domains to package ER into autophagosomes. Lst1-mediated ER-phagy played a vital role in maintaining cellular homeostasis by preventing the accumulation of an aggregation-prone protein in the ER. Lst1 function appears to be conserved because its mammalian homolog, SEC24C, was also required for ER-phagy.

KW - UNFOLDED PROTEIN RESPONSE

KW - SELECTIVE AUTOPHAGY

KW - CARGO

KW - YEAST

KW - LUNAPARK

KW - PATHWAYS

KW - TURNOVER

KW - TRIGGERS

KW - REQUIRES

KW - TEX264

U2 - 10.1126/science.aau9263

DO - 10.1126/science.aau9263

M3 - Article

C2 - 31273116

SN - 0036-8075

VL - 365

SP - 53

EP - 60

JO - Science

JF - Science

IS - 6448

ER -

A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation

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Keywords

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