A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression

Reindert Nijland, Jan-Willem Veening, Oscar P. Kuipers*

*Corresponding author for this work

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Abstract

By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter.

Original languageEnglish
Pages (from-to)2390-2393
Number of pages4
JournalApplied and environmental microbiology
Volume73
Issue number7
DOIs
Publication statusPublished - Apr-2007

Keywords

  • TRANSITION-STATE REGULATOR
  • PROTEIN
  • PHOSPHORELAY
  • INTEGRATION
  • SEQUENCE
  • SIGNALS
  • ABRB

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