A fast and inexpensive DNA extraction/purification protocol for brown macroalgae

Galice Hoarau*, James A. Coyer, Wytze T. Stam, Jeanine L. Olsen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

49 Citations (Scopus)

Abstract

Here we describe a rapid method for extracting DNA from dried brown algae material using a microtitre plate system in conjunction with a milling instrument. The method allows the preparation of nuclear and organelle DNA of quality suitable for polymerase chain reaction amplification. It combines high throughput with low cost per sample: DNA from 192 samples can be extracted in c. 3 h for <EURO0.40 per sample, nearly tenfold cheaper than commercially available kits. Furthermore, by using microtitre plates, efficient storage and downstream processing is facilitated.

Original languageEnglish
Pages (from-to)191-193
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number2
DOIs
Publication statusPublished - Mar-2007

Keywords

  • brown algae
  • DNA extraction
  • heterokonts
  • high throughput
  • Phaeophyceae
  • MICROSATELLITE LOCI
  • FUCUS-VESICULOSUS
  • HETEROKONTOPHYTA
  • PHAEOPHYCEAE
  • SERRATUS
  • SEAWEEDS
  • POPULATION
  • FUCACEAE
  • TISSUE
  • ITS1

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