A fluorescence-based bioassay for antibacterials and its application in screening natural product extracts

Katharina Michels, Ramona Heinke, Pia Schöne, Oscar P Kuipers, Norbert Arnold, Ludger A Wessjohann

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

The reliable assessment of the biological activity of a minor component embedded in a complex matrix of several hundred compounds is a difficult but common task in the search for natural product-based antibiotics, for example, by bioassay-guided fractionation. To quantify the antibiotic properties, it is necessary to assess the cell viability. Direct measurements use CFU counts, OD measurements or detection via fluorescent or reducible dyes. However, natural extracts often already possess intrinsic dye, fluorescent, reducing or protein denaturing properties, or they contain insoluble compounds or general protein-binding (tanning) polyphenols as disturbing features, while at the same time very little of the selective antibiotic sought after is present. A promising alternative is provided by intrinsically produced bright fluorescent proteins. In this paper, a rapid, robust and concentration-dependent assay for screening antibiotics with genetically modified mutants of Bacillus subtilis 168 (PabrB-iyfp) is presented. The Gram-positive bacteria exhibit a native fluorescence during their exponential growth phase due to the expression of improved yellow fluorescent protein. To demonstrate the applicability in the field of natural product research, several compounds and extracts were screened for antibacterial activity, with an emphasis on those from the fungal genus Hygrophorus (waxy caps).The Journal of Antibiotics advance online publication, 8 July 2015; doi:10.1038/ja.2015.71.

Original languageEnglish
Pages (from-to)734-740
Number of pages7
JournalJournal of Antibiotics
Volume68
Issue number12
DOIs
Publication statusPublished - 8-Jul-2015

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