A fluorescent nanodiamond foundation for quantum sensing in cells

Simon Robert Hemelaar

    Research output: ThesisThesis fully internal (DIV)

    744 Downloads (Pure)


    Free radicals play a major role in the aging process as well as a most diseases. However, we barely know anything about them. These tiny molecules have an extremely short lifespan and are difficult to measure, while their role in health related processes is considerable. Fluorescent nanodiamonds are very small diamonds which can shed a light on this research question. These diamonds emit a constant light in a controlled setting. This is possible due to a small defect in the structure of the diamond, which makes it fluorescent. When free radicals are present, the light changes, which allows measurement of the radicals.
    During my PhD I have laid the basis for these biological measurements. Not all cells automatically take up diamonds or the diamonds tend to aggregate in cellular medium. By changing the solutions in which we administer the diamonds, we can prevent these obstacles. I have also performed a very detailed analysis of the cellular response on diamond uptake. Conveniently, the cells hardly show any response to the diamond uptake, an important result for our future measurements. In addition, I have developed new ways of targeting the diamonds to specific places in the cells, to obtain location specific information. Finally I have determined the subcellular location of the diamonds using a new technique, based on integrated electron microscopy. During my work I have laid the foundation for promising cellular research of ageing and disease using fluorescent nanodiamonds!
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • University of Groningen
    • Schirhagl, Romana, Supervisor
    • van Dam, Go, Co-supervisor
    Award date10-Oct-2018
    Place of Publication[Groningen]
    Print ISBNs978-94-034-1014-2
    Electronic ISBNs978-94-034-1013-5
    Publication statusPublished - 2018


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