A Genotypic Approach for Detection, Identification, and Characterization of Drug Resistance in Mycobacterium ulcerans in Clinical Samples and Isolates from Ghana

Marcus Beissner*, Nana Yaa Awua Boateng, William Thompson, Willemien A. Nienhuis, Erasmus Klutse, Pius Agbenorku, Joerg Nitschke, Karl Heinz Herbinger, Vera Siegmund, Erna Fleischmann, Ohene Adiei, Bernhard Fleischer, Tjip S. van der Werf, Thomas Loscher, Gisela Bretzel

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Standardized antimycobacterial therapy is considered the treatment of choice for Buruli ulcer disease To assess the prevalence of drug resistance among clinical Mycobacterium ulcer-ems isolates in Ghana we conducted a sequence based approach to detect mutations associated with drug resistance We subjected clinical samples to direct DNA sequencing of rpoB and rpsL genes and compared culture and whole genome extracts regarding the efficiency of sequence analysis, 99 1% (rpoB) and 100% (rpsL) of the patients harbored M ulcerans wild type In one isolate (0 9%) a point mutation of the rpoB gene at codon Ser522 leading to an amino acid change was detected Culture, extracts yielded a significantly higher sequencing efficiency than whole genome extracts Our data suggest a low level of drug resistance in Ghana However mutations associated with drug resistance do occur and require monitoring Improved techniques are necessary to enhance the efficiency of sequence analysis of whole genome extracts

Original languageEnglish
Pages (from-to)1059-1065
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume83
Issue number5
DOIs
Publication statusPublished - Nov-2010

Keywords

  • POLYMERASE-CHAIN-REACTION
  • DISEASE BURULI ULCER
  • LABORATORY DIAGNOSIS
  • STREPTOMYCIN RESISTANCE
  • GENE
  • TUBERCULOSIS
  • MUTATIONS
  • PCR
  • RIFAMPIN
  • AMPLIFICATION

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