TY - JOUR
T1 - A label-free electrochemical DNA sensor to identify breast cancer susceptibility
AU - Ribovski, Laís
AU - Zucolotto, Valtencir
AU - Janegitz, Bruno C.
N1 - Funding Information:
We gratefully acknowledge the Brazilian funding agencies FAPESP (2015/19099-2) and CNPq (444150/2014-5) for financial support.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Detection of mutations related to cancer predisposition has been an important action in the prevention of cancer and in the cancer genetic profiling. Breast cancer (BRCA) genes mutations are widely known for their relation to genetic predisposition to breast and ovarian cancer. However, the access to genetic tests is still limited. Here, a label-free DNA sensor to detect the mutation 185delAG in the breast cancer 1, early onset (BRCA 1) gene, a founder mutation in the Ashkenazi population, is described. Based on the DNA hybridization and electrochemical impedance spectroscopy (EIS), the sensor was able to distinguish between the mutated and non-mutated sequences, a two-base mismatch. Using a single-strand thiol-modified 25-mer DNA sequence (HS-ssDNA) as probe immobilized in a gold electrode, hybridization studies were performed with [Fe(CN)6]4 − as redox probe and resulted in DNA sensor with a low limit of detection of 0.15 nmol L− 1 in a linear range from 1 to 200 nmol L− 1. This DNA sensor shows prominent electrochemical performance and might be an interesting alternative to distinguish between mutated and non-mutated BRCA1 genes.
AB - Detection of mutations related to cancer predisposition has been an important action in the prevention of cancer and in the cancer genetic profiling. Breast cancer (BRCA) genes mutations are widely known for their relation to genetic predisposition to breast and ovarian cancer. However, the access to genetic tests is still limited. Here, a label-free DNA sensor to detect the mutation 185delAG in the breast cancer 1, early onset (BRCA 1) gene, a founder mutation in the Ashkenazi population, is described. Based on the DNA hybridization and electrochemical impedance spectroscopy (EIS), the sensor was able to distinguish between the mutated and non-mutated sequences, a two-base mismatch. Using a single-strand thiol-modified 25-mer DNA sequence (HS-ssDNA) as probe immobilized in a gold electrode, hybridization studies were performed with [Fe(CN)6]4 − as redox probe and resulted in DNA sensor with a low limit of detection of 0.15 nmol L− 1 in a linear range from 1 to 200 nmol L− 1. This DNA sensor shows prominent electrochemical performance and might be an interesting alternative to distinguish between mutated and non-mutated BRCA1 genes.
KW - BRCA 1 mutation
KW - Breast cancer
KW - DNA sensor
KW - Impedimetric sensor
U2 - 10.1016/j.microc.2017.03.011
DO - 10.1016/j.microc.2017.03.011
M3 - Article
AN - SCOPUS:85015370091
SN - 0026-265X
VL - 133
SP - 37
EP - 42
JO - Microchemical Journal
JF - Microchemical Journal
ER -