A lesion mimic phenotype in tomato obtained by isolating and silencing an Lls1 homologue

S Spassieva*, J Hille

*Corresponding author for this work

Research output: Contribution to journalArticleAcademic

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Abstract

Lesion mimic phenotypes serve as a tool to study the regulation of cell death in plants. In order to obtain a tomato lesion mimic phenotype, we used the conservation of the lethal leaf spot 1 (Lls1) genes between plant species. The tomato Lls1 homologue was cloned, sequenced and analyzed. It showed high conservation at the protein and nucleotide level compared to the genes from maize and Arabidopsis. Using virus induced gene silencing, we obtained a phenotype resembling the lls1 mutant in maize. Lesion formation, lesion spreading and light dependence of the tomato lls1 phenotype are strong indications for the functional conservation of the Lls1 gene between the dicotyledonous tomato and the monocotyledonous maize. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish
Article numberPII S0168-9452(01)00595-7
Pages (from-to)543-549
Number of pages7
JournalPlant Science
Volume162
Issue number4
DOIs
Publication statusPublished - Apr-2002

Keywords

  • lesion mimic phenotypes
  • lethal leaf spot 1
  • programmed cell death
  • virus-induced gene silencing
  • tomato
  • SYSTEMIC ACQUIRED-RESISTANCE
  • PROGRAMMED CELL-DEATH
  • SIGNAL-TRANSDUCTION
  • SALICYLIC-ACID
  • CHLOROPHYLL-B
  • GENE
  • MAIZE
  • PROTEIN
  • MUTANT
  • EXPRESSION

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