A METHOD FOR OBTAINING AXENIC ALGAL CULTURES USING THE ANTIBIOTIC CEFOTAXIME WITH EMPHASIS ON CLADOPHOROPSIS-MEMBRANACEA (CHLOROPHYTA)

W.H.C.F. Kooistra; Stella Boele-Bos; W.T. Stam

doi:10.1111/j.0022-3646.1991.00656.x

A METHOD FOR OBTAINING AXENIC ALGAL CULTURES USING THE ANTIBIOTIC CEFOTAXIME WITH EMPHASIS ON CLADOPHOROPSIS-MEMBRANACEA (CHLOROPHYTA)

W.H.C.F. Kooistra^*, Stella Boele-Bos, W.T. Stam

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Axenic cultures of the tropical green seaweed Cladophoropsis membranacea (C. Agardh) Boergesen were obtained by cutting 2-mm apical tips from fast-growing unialgal filaments and incubating them in 1/2PES containing 100-mu-g.mL-1 cefotaxime. After 1 week, apical tips were cut from the newly grown plantlets, washed through a series of sterile drops of seawater, and incubated in sterile 1/2PES. Plantlets were screened for the presence of bacteria by incubating droplets of the culture medium on peptone agar plates and by examining DAPI-stained filaments. Ninety to one hundred percent of the plantlets obtained with this treatment were axenic. Cefotaxime was also effective against cyanobacteria but of only limited value in rhodophytes.

Original language	English
Pages (from-to)	656-658
Number of pages	3
Journal	Journal of Phycology
Volume	27
Issue number	5
DOIs	https://doi.org/10.1111/j.0022-3646.1991.00656.x
Publication status	Published - Oct-1991

Keywords

ANTIBIOTIC
AXENIC
CEFOTAXIME
CHLOROPHYTA
CYANOBACTERIA
CLADOPHOROPSIS
DAPI
SIPHONOCLADALES
VANCOMYCIN

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A METHOD FOR OBTAINING AXENIC ALGAL CULTURES USING THE ANTIBIOTIC CEFOTAXIME
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title = "A METHOD FOR OBTAINING AXENIC ALGAL CULTURES USING THE ANTIBIOTIC CEFOTAXIME WITH EMPHASIS ON CLADOPHOROPSIS-MEMBRANACEA (CHLOROPHYTA)",

abstract = "Axenic cultures of the tropical green seaweed Cladophoropsis membranacea (C. Agardh) Boergesen were obtained by cutting 2-mm apical tips from fast-growing unialgal filaments and incubating them in 1/2PES containing 100-mu-g.mL-1 cefotaxime. After 1 week, apical tips were cut from the newly grown plantlets, washed through a series of sterile drops of seawater, and incubated in sterile 1/2PES. Plantlets were screened for the presence of bacteria by incubating droplets of the culture medium on peptone agar plates and by examining DAPI-stained filaments. Ninety to one hundred percent of the plantlets obtained with this treatment were axenic. Cefotaxime was also effective against cyanobacteria but of only limited value in rhodophytes.",

keywords = "ANTIBIOTIC, AXENIC, CEFOTAXIME, CHLOROPHYTA, CYANOBACTERIA, CLADOPHOROPSIS, DAPI, SIPHONOCLADALES, VANCOMYCIN",

author = "W.H.C.F. Kooistra and Stella Boele-Bos and W.T. Stam",

year = "1991",

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doi = "10.1111/j.0022-3646.1991.00656.x",

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AU - Kooistra, W.H.C.F.

AU - Boele-Bos, Stella

AU - Stam, W.T.

PY - 1991/10

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N2 - Axenic cultures of the tropical green seaweed Cladophoropsis membranacea (C. Agardh) Boergesen were obtained by cutting 2-mm apical tips from fast-growing unialgal filaments and incubating them in 1/2PES containing 100-mu-g.mL-1 cefotaxime. After 1 week, apical tips were cut from the newly grown plantlets, washed through a series of sterile drops of seawater, and incubated in sterile 1/2PES. Plantlets were screened for the presence of bacteria by incubating droplets of the culture medium on peptone agar plates and by examining DAPI-stained filaments. Ninety to one hundred percent of the plantlets obtained with this treatment were axenic. Cefotaxime was also effective against cyanobacteria but of only limited value in rhodophytes.

AB - Axenic cultures of the tropical green seaweed Cladophoropsis membranacea (C. Agardh) Boergesen were obtained by cutting 2-mm apical tips from fast-growing unialgal filaments and incubating them in 1/2PES containing 100-mu-g.mL-1 cefotaxime. After 1 week, apical tips were cut from the newly grown plantlets, washed through a series of sterile drops of seawater, and incubated in sterile 1/2PES. Plantlets were screened for the presence of bacteria by incubating droplets of the culture medium on peptone agar plates and by examining DAPI-stained filaments. Ninety to one hundred percent of the plantlets obtained with this treatment were axenic. Cefotaxime was also effective against cyanobacteria but of only limited value in rhodophytes.

KW - ANTIBIOTIC

KW - AXENIC

KW - CEFOTAXIME

KW - CHLOROPHYTA

KW - CYANOBACTERIA

KW - CLADOPHOROPSIS

KW - DAPI

KW - SIPHONOCLADALES

KW - VANCOMYCIN

U2 - 10.1111/j.0022-3646.1991.00656.x

DO - 10.1111/j.0022-3646.1991.00656.x

M3 - Article

SN - 0022-3646

VL - 27

SP - 656

EP - 658

JO - Journal of Phycology

JF - Journal of Phycology

IS - 5

ER -

A METHOD FOR OBTAINING AXENIC ALGAL CULTURES USING THE ANTIBIOTIC CEFOTAXIME WITH EMPHASIS ON CLADOPHOROPSIS-MEMBRANACEA (CHLOROPHYTA)

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Keywords

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