A mouse model for spatial and temporal expression of HGF in the heart

Ilan Riess, Valentina Sala, Christian Leo, Marco Demaria, Stefano Gatti, Simona Gallo, Amandine Fitou, Ombretta Boero, Renzo Levi, Ivan Cuccovillo, Fabiola Molla, Noeleen De Angelis, Lidia Staszewsky, Roberto Latini, Tiziana Crepaldi

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8 Citations (Scopus)


In order to study the effects of Hepatocyte Growth Factor (HGF) in the heart, two transgenic mice were developed, one carrying a bidirectional HGF-TetO-GFP responder construct and the other carrying a α-MHC-tTA transactivator construct. Crosses were carried out between heterozygotes, so that litters contained bitransgenic α-MHC-tTA/HGF-TetO-GFP+, thus expressing HGF and GFP exclusively in the heart and only in the absence of Doxycycline. Our data show that the expression of HGF was indeed restricted to the heart and that the expression was limited to the timeframe of the absence of Doxycycline. Surprisingly the expression was variable even between bitransgenic littermates. In the setting of a model of ischemia-reperfusion, the expression of HGF ameliorates cardiac functionality, enhances proliferation and diminishes the scarred area, proving that this is a good model to study the beneficial influences and functional roles of HGF in the heart.

Original languageEnglish
Pages (from-to)1203-16
Number of pages14
JournalTransgenic Research
Issue number6
Publication statusPublished - Dec-2011
Externally publishedYes


  • Animals
  • Blotting, Western
  • Cell Line
  • Cell Proliferation
  • Collagen
  • Crosses, Genetic
  • Culture Media, Conditioned
  • Dogs
  • Doxycycline
  • Echocardiography
  • Female
  • Gene Expression Regulation
  • Green Fluorescent Proteins
  • Heart
  • Hepatocyte Growth Factor
  • Heterozygote
  • Immunohistochemistry
  • Mice
  • Mice, Transgenic
  • Models, Animal
  • Myocardial Reperfusion Injury
  • Pregnancy
  • Real-Time Polymerase Chain Reaction

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