TY - JOUR
T1 - A novel approach to ion separations in solution
T2 - synchronized cyclic capillary electrophoresis (SCCE)
AU - Burggraf, Norbert
AU - Manz, Andreas
AU - Verpoorte, Elisabeth
AU - Effenhauser, Carlo S.
AU - Michael Widmer, H.
AU - de Rooij, Nico F.
PY - 1994/6
Y1 - 1994/6
N2 - A novel concept for ion separation is presented. Repeated column switching in capillary electrophoresis allows for the elimination of unwanted sample components, and for the separation of species having very similar mobilities. Theoretical considerations predict good separation efficiencies, e.g., high plate numbers per volt. Using micromachining techniques, a planar glass structure has been fabricated, composed of four capillaries of 20 mm length arranged in a square. Laser fluorescence detection is used in the detection scheme. To move a component around one cycle, 1 min is needed when applying 2.5 kV. A demonstration of the peak band broadening after several cycles and a separation example are given.
AB - A novel concept for ion separation is presented. Repeated column switching in capillary electrophoresis allows for the elimination of unwanted sample components, and for the separation of species having very similar mobilities. Theoretical considerations predict good separation efficiencies, e.g., high plate numbers per volt. Using micromachining techniques, a planar glass structure has been fabricated, composed of four capillaries of 20 mm length arranged in a square. Laser fluorescence detection is used in the detection scheme. To move a component around one cycle, 1 min is needed when applying 2.5 kV. A demonstration of the peak band broadening after several cycles and a separation example are given.
UR - http://www.scopus.com/inward/record.url?scp=0028442531&partnerID=8YFLogxK
U2 - 10.1016/0925-4005(93)01200-N
DO - 10.1016/0925-4005(93)01200-N
M3 - Article
AN - SCOPUS:0028442531
SN - 0925-4005
VL - 20
SP - 103
EP - 110
JO - Sensors and Actuators: B. Chemical
JF - Sensors and Actuators: B. Chemical
IS - 2-3
ER -