A novel Tn1696-like composite transposon (Tn6404) harboring bla(IMP-4) in a Klebsiella pneumoniae isolate carrying a rare ESBL gene bla(SFO-1)

Kai Zhou, Wei Yu, Ping Shen, Haifeng Lu, Baohong Wang, John W. A. Rossen, Yonghong Xiao*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Genetic determinants of a clinical Klebsiella pneumoniae isolate (KP1814) coproducing IMP-4 and a rare ESBL gene SFO-1 was investigated. KP1814 belongs to a novel sequence type (ST) assigned to ST2270. WGS identified four circular DNA sequences in KP1814, including two multidrug-resistance (MDR) plasmids, one virulence plasmid, and one circular form. The MDR plasmid pKP1814-1 (299.9 Kb) is untypeable, and carries two large mosaic multiresistance regions (MRRs). bla(SFO-1) and bla(IMP-4) co-exists on MRR1, and bla(SFO-1) is associated with an IS/Tn-independent genetic context. bla(IMP-4) is carried by a novel In804-like integron (intlI-bla(IMP-4)-Kl.pn.I3-qacG2-aacA4-catB3 Delta) associated with a novel Tn1696-like transposon (designed Tn6404) flanked by IS5075. The other MDR plasmid pKP1814-3 is a 95,701-bp IncFII plasmid, and is a hybrid of a Shigella flexneri plasmid pSF07201 and an E. coli plasmid pCA08. All resistance genes of pKP1814-3 were detected in a similar to 16-kb IS26-flanked composite transposon carried by a Tn5396 transposon. The circular form (18.3 Kb) was composed of two parts belonging to pKP1814-1 and pKP1814-3, respectively. The plasmid pKP1814-2, carrying multiple virulence factors, encodes IncFIB(K) and IncFII(K) replicons with a size of 187,349 bp. The coexistence of MDR and virulence plasmids largely enhances the bacterial fitness in the host and environment.

Original languageEnglish
Article number17321
Number of pages7
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 11-Dec-2017

Keywords

  • METALLO-BETA-LACTAMASE
  • ESCHERICHIA-COLI
  • CONJUGATIVE PLASMID
  • NUCLEOTIDE-SEQUENCE
  • BIOFILM FORMATION
  • HIGH PREVALENCE
  • CHINA
  • IMP-4
  • RESISTANCE
  • AUSTRALIA

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