Abstract
Genetic determinants of a clinical Klebsiella pneumoniae isolate (KP1814) coproducing IMP-4 and a rare ESBL gene SFO-1 was investigated. KP1814 belongs to a novel sequence type (ST) assigned to ST2270. WGS identified four circular DNA sequences in KP1814, including two multidrug-resistance (MDR) plasmids, one virulence plasmid, and one circular form. The MDR plasmid pKP1814-1 (299.9 Kb) is untypeable, and carries two large mosaic multiresistance regions (MRRs). bla(SFO-1) and bla(IMP-4) co-exists on MRR1, and bla(SFO-1) is associated with an IS/Tn-independent genetic context. bla(IMP-4) is carried by a novel In804-like integron (intlI-bla(IMP-4)-Kl.pn.I3-qacG2-aacA4-catB3 Delta) associated with a novel Tn1696-like transposon (designed Tn6404) flanked by IS5075. The other MDR plasmid pKP1814-3 is a 95,701-bp IncFII plasmid, and is a hybrid of a Shigella flexneri plasmid pSF07201 and an E. coli plasmid pCA08. All resistance genes of pKP1814-3 were detected in a similar to 16-kb IS26-flanked composite transposon carried by a Tn5396 transposon. The circular form (18.3 Kb) was composed of two parts belonging to pKP1814-1 and pKP1814-3, respectively. The plasmid pKP1814-2, carrying multiple virulence factors, encodes IncFIB(K) and IncFII(K) replicons with a size of 187,349 bp. The coexistence of MDR and virulence plasmids largely enhances the bacterial fitness in the host and environment.
Original language | English |
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Article number | 17321 |
Number of pages | 7 |
Journal | Scientific Reports |
Volume | 7 |
DOIs | |
Publication status | Published - 11-Dec-2017 |
Keywords
- METALLO-BETA-LACTAMASE
- ESCHERICHIA-COLI
- CONJUGATIVE PLASMID
- NUCLEOTIDE-SEQUENCE
- BIOFILM FORMATION
- HIGH PREVALENCE
- CHINA
- IMP-4
- RESISTANCE
- AUSTRALIA