A PCR amplification strategy for unrestricted generation of chimeric genes

Michel J. Vos, Harm H. Kampinga*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    4 Citations (Scopus)

    Abstract

    For analyzing protein function, protein dynamics, or protein-protein interactions, the use of chimeric proteins has become an indispensable tool. The generation of DNA constructs coding for such fused proteins can, however, be a tedious process. Currently used strategies often make use of available endonuclease sites, leading to limitations in the choice of the site of fusion between two genes and problems in maintaining protein secondary structure. We have developed a cloning strategy to get around these disadvantages that is based on a single round of PCR amplification followed by antibiotic-resistant gene complementation. (C) 2008 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)338-340
    Number of pages3
    JournalAnalytical Biochemistry
    Volume380
    Issue number2
    DOIs
    Publication statusPublished - 15-Sep-2008

    Keywords

    • PROTEINS

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