A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

Timo Koopmans; Frank J. Dekker; Nathaniel I. Martin

doi:10.1039/c2ra20082a

A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

Timo Koopmans
, Frank J. Dekker
, Nathaniel I. Martin^*

^*Corresponding author for this work

Biopharmaceuticals, Discovery, Design and Delivery (BDDD)

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecules from streptavidin resin.

Original language	English
Pages (from-to)	2244-2246
Number of pages	3
Journal	RSC Advances
Volume	2
Issue number	6
DOIs	https://doi.org/10.1039/c2ra20082a
Publication status	Published - 2012

Keywords

SELECTIVE ENRICHMENT
CHEMICAL PROTEOMICS
CONTAINING PEPTIDES
CYCLOADDITION
REPORTERS
LIGANDS
PROBES

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A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules
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title = "A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules",

abstract = "A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecules from streptavidin resin.",

keywords = "SELECTIVE ENRICHMENT, CHEMICAL PROTEOMICS, CONTAINING PEPTIDES, CYCLOADDITION, REPORTERS, LIGANDS, PROBES",

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year = "2012",

doi = "10.1039/c2ra20082a",

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pages = "2244--2246",

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AU - Dekker, Frank J.

AU - Martin, Nathaniel I.

PY - 2012

Y1 - 2012

N2 - A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecules from streptavidin resin.

AB - A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecules from streptavidin resin.

KW - SELECTIVE ENRICHMENT

KW - CHEMICAL PROTEOMICS

KW - CONTAINING PEPTIDES

KW - CYCLOADDITION

KW - REPORTERS

KW - LIGANDS

KW - PROBES

U2 - 10.1039/c2ra20082a

DO - 10.1039/c2ra20082a

M3 - Article

SN - 2046-2069

VL - 2

SP - 2244

EP - 2246

JO - RSC Advances

JF - RSC Advances

IS - 6

ER -

A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

Abstract

Keywords

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