A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy

OV Stasyk, IJ van der Klei, AR Bellu, SG Shen, JAKW Kiel, JM Cregg, M Veenhuis*, Oleh V. Stasyk, Shigang Shen, James M. Cregg

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Methylotrophic yeasts contain large peroxisomes during growth on methanol. Upon exposure to excess glucose or ethanol these organelles are selectively degraded by autophagy, Here we describe the cloning of a Pichia pastoris gene (PpVPS15) involved ill peroxisome degradation, which is homologous to Saccharomyces cerevisiae VPS15. In methanol-grown cells of a P. pastoris VPS15 deletion strain, the levels of peroxisomal marker enzymes remained high after addition of excess glucose or ethanol. Electron microscopic studies revealed that the organelles were not taken up by vacuoles, suggesting that PpVPS15 is required at an early stage in peroxisome degradation.

Original languageEnglish
Pages (from-to)262-269
Number of pages8
JournalCurrent Genetics
Volume36
Issue number5
DOIs
Publication statusPublished - Nov-1999

Keywords

  • Pichia pastoris
  • peroxisome degradation
  • autophagy
  • VPS15
  • YEAST HANSENULA-POLYMORPHA
  • LYSOSOME-LIKE VACUOLE
  • PROTEIN-KINASE
  • ALCOHOL OXIDASE
  • METHYLOTROPHIC YEASTS
  • MATRIX PROTEIN
  • DEGRADATION
  • BIOGENESIS
  • GENE
  • 3-KINASE

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