A plasmid from the methylotrophic actinomycete amycolatopsis-methanolica capable of site-specific integration

JW VRIJBLOED; J MADON; L DIJKHUIZEN

A plasmid from the methylotrophic actinomycete amycolatopsis-methanolica capable of site-specific integration

JW VRIJBLOED, J MADON, L DIJKHUIZEN

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

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Abstract

Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.

Original language	English
Pages (from-to)	7087-7090
Number of pages	4
Journal	Journal of Bacteriology
Volume	176
Issue number	22
Publication status	Published - Nov-1994

Keywords

STREPTOMYCES-LIVIDANS
NOCARDIA-MEDITERRANEI
SACCHAROPOLYSPORA-ERYTHRAEA
STRUCTURAL-ANALYSIS
GENETIC ELEMENTS
DNA-SEQUENCES
EXCISION
PSAM2
SLP1
SIMILARITIES

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A PLASMID FROM THE METHYLOTROPHIC ACTINOMYCETE AMYCOLATOPSIS-METHANOLICA CAPABLE OF SITE-SPECIFIC INTEGRATION

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@article{3565d2ee71154b489b6bbbc3de7a48f6,

title = "A plasmid from the methylotrophic actinomycete amycolatopsis-methanolica capable of site-specific integration",

abstract = "Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.",

keywords = "STREPTOMYCES-LIVIDANS, NOCARDIA-MEDITERRANEI, SACCHAROPOLYSPORA-ERYTHRAEA, STRUCTURAL-ANALYSIS, GENETIC ELEMENTS, DNA-SEQUENCES, EXCISION, PSAM2, SLP1, SIMILARITIES",

author = "JW VRIJBLOED and J MADON and L DIJKHUIZEN",

year = "1994",

month = nov,

language = "English",

volume = "176",

pages = "7087--7090",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "AMER SOC MICROBIOLOGY",

number = "22",

}

TY - JOUR

T1 - A plasmid from the methylotrophic actinomycete amycolatopsis-methanolica capable of site-specific integration

AU - VRIJBLOED, JW

AU - MADON, J

AU - DIJKHUIZEN, L

PY - 1994/11

Y1 - 1994/11

N2 - Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.

AB - Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.

KW - STREPTOMYCES-LIVIDANS

KW - NOCARDIA-MEDITERRANEI

KW - SACCHAROPOLYSPORA-ERYTHRAEA

KW - STRUCTURAL-ANALYSIS

KW - GENETIC ELEMENTS

KW - DNA-SEQUENCES

KW - EXCISION

KW - PSAM2

KW - SLP1

KW - SIMILARITIES

M3 - Comment/Letter to the editor

SN - 0021-9193

VL - 176

SP - 7087

EP - 7090

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 22

ER -

A plasmid from the methylotrophic actinomycete amycolatopsis-methanolica capable of site-specific integration

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