A role for intestinal alkaline phosphatase in the maintenance of local gut immunity

K.T. Chen, M. Malo, L.K. Beasley-Topliffe, K. Poelstra, J. Millan, G. Mostafa, S. Alam, S. Ramasamy, H. Warren, E. Hohmann, R.A. Hodin

Research output: Contribution to journalMeeting AbstractAcademic

Abstract

Background and Aims: Intestinal alkaline phosphatase (IAP) is a gut mucosal defense factor known to dephosphorylate lipopolysaccharide (LPS); however, the role of IAP in the gut response to luminal bacteria remains undefined. We investigated immune responses of wildtype (WT) and IAP-knockout (IAP-KO) mice to LPS and Salmonella typhimurium challenges. Methods: Cryotstat sectioning and standard indirect immunohistochemical staining for MHC Class II molecules were performed on liver tissue from WT and IAP-KO mice. WT and IAP-KO mice were orally gavaged with S typhimurium; after 6 days, bacterial translocation to mesenteric nodes, liver and spleen was determined by plating. WT and IAP-KO mice received intraperitoneal injections of LPS, with subsequent quantification of complete blood counts and serum Il-6 by ELISA. WT and IAPKO whole blood were plated and stimulated with LPS and Pam-3-Cys, with Il-6 determination. Results: Immunohistologic liver exams showed increased expression of MHC Class II molecules in IAP-KO mice. Following S typhimurium challenge, WT mice appeared moribund compared to IAP-KO mice, with increased bacterial translocation. WT mice had >50% decrease (P
Original languageEnglish
Pages (from-to)258-259
Number of pages2
JournalJournal of Surgical Research
Volume158
Issue number2
DOIs
Publication statusPublished - 1-Feb-2010

Keywords

  • alkaline phosphatase
  • lipopolysaccharide
  • interleukin 6
  • intestine
  • immunity
  • surgery
  • society
  • university
  • surgeon
  • human
  • mouse
  • knockout mouse
  • liver
  • blood
  • Salmonella enterica subsp. enterica serovar Typhimurium
  • serum
  • bacterial translocation
  • dying
  • immune response
  • staining
  • blood cell count
  • intestine epithelium
  • commensal
  • intraperitoneal drug administration
  • spleen
  • injection
  • thrombocyte
  • stimulation
  • secretion (process)
  • microflora
  • Bacteria
  • enzyme linked immunosorbent assay

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