A sensor for intracellular ionic strength

Esther Biemans-Oldehinkel, Nik A.B.N. Mahmood, Bert Poolman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademic

100 Citations (Scopus)
461 Downloads (Pure)

Abstract

Cystathionine-β-synthase (CBS) domains are found in >4,000 proteins in species from all kingdoms of life, yet their functions are largely unknown. Tandem CBS domains are associated with membrane transport proteins, most notably members of the ATP-binding cassette (ABC) superfamily; voltage-gated chloride channels and transporters; cation efflux systems; and various enzymes, transcription factors, and proteins of unknown function. We now show that tandem CBS domains in the osmoregulatory ABC transporter OpuA are sensors for ionic strength that control the transport activity through an electrostatic switching mechanism. The on/off state of the transporter is determined by the surface charge of the membrane and the internal ionic strength that is sensed by the CBS domains. By modifying the CBS domains, we can control the ionic strength dependence of the transporter: deleting a stretch of C-terminal anionic residues shifts the ionic strength dependence to higher values, whereas deleting the CBS domains makes the system largely independent of ionic strength. We present a model for the gating of membrane transport by ionic strength and propose a new role for CBS domains.
Original languageEnglish
Pages (from-to)10624 - 10629
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume103
Issue number28
DOIs
Publication statusPublished - 2006

Keywords

  • osmoregulation
  • ionic strength sensor
  • cell volume regulation
  • cystathionine-β-synthase domains
  • ATP-binding cassette transporter

Cite this