A sensor for quantification of macromolecular crowding in living cells

Arnold J. Boersma*, Inge S. Zuhorn, Bert Poolman

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

122 Citations (Scopus)

Abstract

Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.

Original languageEnglish
Pages (from-to)227-229
Number of pages3
JournalNature Methods
Volume12
Issue number3
DOIs
Publication statusPublished - Mar-2015

Keywords

  • PROTEIN STABILITY
  • LIVE CELLS
  • IN-VIVO
  • CYTOPLASM
  • CONSEQUENCES
  • CONFINEMENT
  • DIFFUSION
  • FICOLL

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