A Study on the Effects of IL-10 in Anti-Thy 1-Induced Glomerulonephritis in Rats

H. Rachmawati*, L. Beljaars, C. Reker-Smit, H. I. Bakker, A. M. Van Loenen-Weemaes, K. Poelstra

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In the present study, we examined the effects of IL-10 after 24 h in a model of acute glomerulonephritis (GN). One hour after the anti-Thy I antibody administration, a single i.v. dose of IL-10 was administered to rats. Normal rats, control nephritic rats and nephritic rats treated with IL-10, were sacrificed 24 h after administration of antibody. Samples of urine, blood and organs were subsequently collected. The effects of IL-10 were studied by quantification of various inflammatory parameters at the protein level after immunohistochemical staining and at the mRNA level by a quantitative real time PCR technique. Nitric oxide and protein content were determined in serum and in 24 h-excreted urine, respectively. The inflammatory parameters were reduced in the IL-10-treated group: in increment in glomerular CD14, ICAM-1 and MMP-13 staining induced by anti-Thy 1 injection was significantly attenuated by IL-10. In contrast, mRNA levels for CD14, IL-1 beta, TNF-alpha and MCP-1 were not different between IL-10-treated and control GN groups. In conclusion, a single i.v. dose of IL-10 suppresses the expression of several inflammatory parameters, 24 h after inducing of acute GN but at this time point mRNA levels of all parameters examined were not affected. Although its therapeutic efficacy needs further evaluation, anti-inflammatory effects of this short-lived cytokine can be found one day after its administration.

Original languageEnglish
Pages (from-to)346-353
Number of pages8
JournalInternational journal of pharmacology
Volume5
Issue number6
Publication statusPublished - 2009

Keywords

  • IL-10
  • glomerulonephritis
  • anti-Thy1
  • inflammation
  • immunohistochemistry
  • MESANGIAL PROLIFERATIVE GLOMERULONEPHRITIS
  • TUMOR-NECROSIS-FACTOR
  • MATRIX METALLOPROTEINASES
  • IGA NEPHROPATHY
  • MEMBRANOUS NEPHROPATHY
  • MACROPHAGE ACTIVATION
  • INTERLEUKIN-10
  • NEPHRITIS
  • LIPOPOLYSACCHARIDE
  • CELLS

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