A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment

Abdulfataah A.A. Mohamed; Peter Walland; Jasper Stevens; Marco van Londen; Hiddo J.L. Heerspink; Ron T. Gansevoort; Nico C. van de Merbel

doi:10.1016/j.jchromb.2024.124329

A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment

Abdulfataah A.A. Mohamed, Peter Walland, Jasper Stevens, Marco van Londen, Hiddo J.L. Heerspink, Ron T. Gansevoort, Nico C. van de Merbel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Downloads (Pure)

Abstract

A novel liquid chromatography-tandem mass spectrometry method is described for the quantitative determination of the kidney function markers iothalamate and hippuran in human serum and urine. It is based on protein precipitation with methanol followed by dilution of the supernatant for serum and simple dilution for urine. The polar analytes are chromatographically separated by a 6.5-min gradient on a low-ligand density reversed-phase column; detection is performed by electrospray ionization tandem mass spectrometry in the positive ion mode against stable-isotope labeled internal standards.

The results of a thorough method validation show that iothalamate and hippuran can be simultaneously quantified in the concentration ranges 0.500–30.0 ng/mL and 10.0–5000 ng/mL for serum and urine, respectively, with values for CV and absolute bias not exceeding 10 %, and with sufficient stability in all relevant matrices and solvents. The method was successfully applied for the analysis of serum and urine samples of multiple individuals who received both iothalamate and hippuran.

Original language	English
Article number	124329
Number of pages	8
Journal	Journal of Chromatography B
Volume	1247
DOIs	https://doi.org/10.1016/j.jchromb.2024.124329
Publication status	Published - Oct-2024

Keywords

IOTHALAMATE
Hippuran
exogenous filtration marker
non-radioactive
kidney function
mGFR
ERPF
LC-MS/MS
validation of a method

Access to Document

10.1016/j.jchromb.2024.124329Licence: CC BY

Mohamed et al_ LCMSMS iothalamate and hippuran serum urine_JChromB_2024
1570-0232/© 2024 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Final publisher's version, 1.6 MBLicence: CC BY

Handle.net

Persistent link

Cite this

Mohamed, A. A. A., Walland, P., Stevens, J., van Londen, M., Heerspink, H. J. L., Gansevoort, R. T., & van de Merbel, N. C. (2024). A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment. Journal of Chromatography B, 1247, Article 124329. https://doi.org/10.1016/j.jchromb.2024.124329

@article{bbc05c3d379f4abeaaab5b343ba4bad7,

title = "A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment",

abstract = "A novel liquid chromatography-tandem mass spectrometry method is described for the quantitative determination of the kidney function markers iothalamate and hippuran in human serum and urine. It is based on protein precipitation with methanol followed by dilution of the supernatant for serum and simple dilution for urine. The polar analytes are chromatographically separated by a 6.5-min gradient on a low-ligand density reversed-phase column; detection is performed by electrospray ionization tandem mass spectrometry in the positive ion mode against stable-isotope labeled internal standards.The results of a thorough method validation show that iothalamate and hippuran can be simultaneously quantified in the concentration ranges 0.500–30.0 ng/mL and 10.0–5000 ng/mL for serum and urine, respectively, with values for CV and absolute bias not exceeding 10 %, and with sufficient stability in all relevant matrices and solvents. The method was successfully applied for the analysis of serum and urine samples of multiple individuals who received both iothalamate and hippuran.",

keywords = "IOTHALAMATE, Hippuran, exogenous filtration marker, non-radioactive, kidney function, mGFR, ERPF, LC-MS/MS, validation of a method",

author = "Mohamed, {Abdulfataah A.A.} and Peter Walland and Jasper Stevens and {van Londen}, Marco and Heerspink, {Hiddo J.L.} and Gansevoort, {Ron T.} and {van de Merbel}, {Nico C.}",

year = "2024",

month = oct,

doi = "10.1016/j.jchromb.2024.124329",

language = "English",

volume = "1247",

journal = "Journal of Chromatography B",

issn = "1570-0232",

publisher = "ELSEVIER SCIENCE BV",

}

TY - JOUR

T1 - A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment

AU - Mohamed, Abdulfataah A.A.

AU - Walland, Peter

AU - Stevens, Jasper

AU - van Londen, Marco

AU - Heerspink, Hiddo J.L.

AU - Gansevoort, Ron T.

AU - van de Merbel, Nico C.

PY - 2024/10

Y1 - 2024/10

N2 - A novel liquid chromatography-tandem mass spectrometry method is described for the quantitative determination of the kidney function markers iothalamate and hippuran in human serum and urine. It is based on protein precipitation with methanol followed by dilution of the supernatant for serum and simple dilution for urine. The polar analytes are chromatographically separated by a 6.5-min gradient on a low-ligand density reversed-phase column; detection is performed by electrospray ionization tandem mass spectrometry in the positive ion mode against stable-isotope labeled internal standards.The results of a thorough method validation show that iothalamate and hippuran can be simultaneously quantified in the concentration ranges 0.500–30.0 ng/mL and 10.0–5000 ng/mL for serum and urine, respectively, with values for CV and absolute bias not exceeding 10 %, and with sufficient stability in all relevant matrices and solvents. The method was successfully applied for the analysis of serum and urine samples of multiple individuals who received both iothalamate and hippuran.

AB - A novel liquid chromatography-tandem mass spectrometry method is described for the quantitative determination of the kidney function markers iothalamate and hippuran in human serum and urine. It is based on protein precipitation with methanol followed by dilution of the supernatant for serum and simple dilution for urine. The polar analytes are chromatographically separated by a 6.5-min gradient on a low-ligand density reversed-phase column; detection is performed by electrospray ionization tandem mass spectrometry in the positive ion mode against stable-isotope labeled internal standards.The results of a thorough method validation show that iothalamate and hippuran can be simultaneously quantified in the concentration ranges 0.500–30.0 ng/mL and 10.0–5000 ng/mL for serum and urine, respectively, with values for CV and absolute bias not exceeding 10 %, and with sufficient stability in all relevant matrices and solvents. The method was successfully applied for the analysis of serum and urine samples of multiple individuals who received both iothalamate and hippuran.

KW - IOTHALAMATE

KW - Hippuran

KW - exogenous filtration marker

KW - non-radioactive

KW - kidney function

KW - mGFR

KW - ERPF

KW - LC-MS/MS

KW - validation of a method

U2 - 10.1016/j.jchromb.2024.124329

DO - 10.1016/j.jchromb.2024.124329

M3 - Article

SN - 1570-0232

VL - 1247

JO - Journal of Chromatography B

JF - Journal of Chromatography B

M1 - 124329

ER -

A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment

Abstract

Keywords

Access to Document

Handle.net

Fingerprint

Cite this