Absence of mutations in the RET gene in acute myeloid leukemia

M Visser*, RMW Hofstra, RP Stulp, Y Wu, CHCM Buys, R Willemze, JE Landegent

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)

    Abstract

    Expression of the tyrosine kinase receptor RET has previously been detected in normal hematopoietic cells, and especially in cells of the myeloid lineage. Furthermore, RET was shown to be differentially expressed in acute myeloid leukemia (AML), a disease characterized by excessive cell growth and aberrant maturation of cells, with the highest levels of expression in leukemias with monocytic differentiation. RET is known to be expressed in cells from the excretory system and from the developing central and peripheral nervous system. Both activating and inactivating aberrations in the RET gene have been detected in disorders derived from these tissues. To investigate whether the differential expression is a primary defect in AML, the presence of RET alterations was scanned by Southern blot analysis on DNA of blasts obtained from 17 AML patients. However, no RET gene aberrations were found. Subsequently, denaturing gradient gel electrophoresis (DGGE) analysis was performed on the DNA. of blasts from ten selected cases. All five variants detected turned out to represent neutral DNA polymorphisms, including a novel polymorphism in exon 14. Since we were unable to detect mutations of RET in AML, it is unlikely that it plays an important role in leukemogenesis.

    Original languageEnglish
    Pages (from-to)87-90
    Number of pages4
    JournalAnnals of Hematology
    Volume75
    Issue number3
    Publication statusPublished - Sep-1997

    Keywords

    • hematopoiesis
    • AML
    • RET
    • mutation analysis
    • GRADIENT GEL-ELECTROPHORESIS
    • ACUTE MYELOBLASTIC-LEUKEMIA
    • TYROSINE KINASE
    • TRANSFORMING GENE
    • PROTEIN PRODUCT
    • CSF-1 RECEPTOR
    • C-KIT
    • PROTOONCOGENE
    • EXPRESSION
    • ACTIVATION

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