Absolute beta-catenin concentrations in Wnt pathway-stimulated and non-stimulated cells

S Sievers, C Fritzsch, M Grzegorczyk, C Kuhnen, O Muller*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

The intracellular level of the proto-oncoprotein beta-catenin is a parameter for the activity of the Wnt pathway, which has been linked to carcinogenesis. The paper introduces a novel sandwich-based ELISA for the determination of the beta-catenin concentration in lysates from cells or tissues. The advantages of the method were proven by determining beta-catenin levels in cell lines and in cells after activation of the Wnt pathway. Analysis revealed high beta-catenin concentrations in the cell lines HeLa, KB, HT1080, MCF-7, U-87 and U-373, which had not been described before. beta-Catenin concentrations were compared in HEK293 and C57MG cells after activation of the Wnt pathway. The beta-catenin concentrations increased by different factors depending on whether the Wnt pathway was activated by incubation with LiCl or with Wnt-3a-conditioned medium. This finding indicated that the beta-catenin level depends on the way and level of Wnt pathway activation. The quantitative analysis of beta-catenin in colorectal tumours revealed high beta-catenin levels in tumours with truncating mutations in the APC gene.

Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalBiomarkers
Volume11
Issue number3
DOIs
Publication statusPublished - 2006

Keywords

  • beta-catenin
  • ELISA
  • Wnt pathway
  • diagnostic marker
  • HUMAN COLORECTAL-CANCER
  • CARCINOMAS
  • MUTATIONS
  • PROTEIN
  • EXPRESSION
  • FREQUENT
  • GENE
  • LINES
  • APC
  • ACCUMULATION

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