ACTIVATION OF G-PROTEINS BY RECEPTOR-STIMULATED NUCLEOSIDE DIPHOSPHATE KINASE IN DICTYOSTELIUM

Anthony A. Bominaar, Anco C. Molijn, Martine Pestel, Michel Veron, Peter J.M. van Haastert

Research output: Contribution to journalArticleAcademicpeer-review

69 Citations (Scopus)
192 Downloads (Pure)

Abstract

Recently, interest in the enzyme nucleoside diphosphate kinase (EC 2.7.4.6) has increased as a result of its possible involvement in cell proliferation and development. Since NDP kinase is one of the major sources of GTP in cells, it has been suggested that the effects of an altered NDP kinase activity on cellular processes might be the result of altered transmembrane signal transduction via guanine nucleotide-binding proteins (G-proteins). In the cellular slime mould Dictyostelium discoideum, extracellular cAMP induces an increase of phospholipase C activity via a surface cAMP receptor and G-proteins. In this paper it is demonstrated that part of the cellular NDP kinase is associated with the membrane and stimulated by cell surface cAMP receptors. The GTP produced by the action of NDP kinase is capable of activating G-proteins as monitored by altered G-protein-receptor interaction and the activation of the effector enzyme phospholipase C. Furthermore, specific monoclonal antibodies inhibit the effect of NDP kinase on G-protein activation. These results suggest that receptor-stimulated NDP kinase contributes to the mediation of hormone action by producing GTP for the activation of GTP-binding proteins.

Original languageEnglish
Pages (from-to)2275-2279
Number of pages5
JournalEMBO Journal
Volume12
Issue number6
Publication statusPublished - Jun-1993

Keywords

  • DICTYOSTELIUM-DISCOIDEUM
  • G-PROTEINS
  • NDP KINASE
  • PHOSPHOLIPASE-C
  • SURFACE RECEPTORS
  • GTP-BINDING-PROTEINS
  • TUMOR-METASTASIS
  • DROSOPHILA DEVELOPMENT
  • GUANINE-NUCLEOTIDES
  • DISCOIDEUM
  • CELLS
  • NM23
  • MEMBRANES
  • GENE

Cite this