Acute glomerulonephritis after intravenous injection of monoclonal anti-thymocyte antibodies in the rat

W. M. Baghus; P. J. Hoedemaeker; J. Rozing; W. W. Bakker

doi:10.1016/0165-2478(86)90091-X

Acute glomerulonephritis after intravenous injection of monoclonal anti-thymocyte antibodies in the rat

W. M. Baghus^*, P. J. Hoedemaeker, J. Rozing, W. W. Bakker

^*Corresponding author for this work

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Abstract

Female Wistar rats were injected with (mouse) monoclonal antibodies (Moabs) of different IgG subclasses directed to rat thymocytes or rat tumor cells. Following intravenous injection of antithymocyte Moabs, glomerular binding of mouse IgG was observed during the first 4 days along the GBM and in the mesangium. No staining for mouse IgG was detected in anti-tumor Moab injected rats. Animals injected with IgG 2a anti-thymocyte Moab developed glomerulonephritis and a massive proteinuria in contrast to rats injected with IgG 1 Moab which is non-complement fixing. The glomerulonephritis lesion consisted of microaneurysms and focal and segmental proliferation. Deposits of complement and fibrin could be detected exclusively in rats injected with IgG 2a anti-thymocyte Moab during the whole observation period of 14 days. This is the first demonstration of overt glomerulonephritis lesions on the injection of monoclonal antibodies

Original language	English
Pages (from-to)	109-113
Number of pages	5
Journal	Immunology Letters
Volume	12
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-2478(86)90091-X
Publication status	Published - Mar-1986

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Acute glomerulonephritis after intravenous injection of monoclonal anti-thymocyte antibodies in the rat
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title = "Acute glomerulonephritis after intravenous injection of monoclonal anti-thymocyte antibodies in the rat",

abstract = "Female Wistar rats were injected with (mouse) monoclonal antibodies (Moabs) of different IgG subclasses directed to rat thymocytes or rat tumor cells. Following intravenous injection of antithymocyte Moabs, glomerular binding of mouse IgG was observed during the first 4 days along the GBM and in the mesangium. No staining for mouse IgG was detected in anti-tumor Moab injected rats. Animals injected with IgG 2a anti-thymocyte Moab developed glomerulonephritis and a massive proteinuria in contrast to rats injected with IgG 1 Moab which is non-complement fixing. The glomerulonephritis lesion consisted of microaneurysms and focal and segmental proliferation. Deposits of complement and fibrin could be detected exclusively in rats injected with IgG 2a anti-thymocyte Moab during the whole observation period of 14 days. This is the first demonstration of overt glomerulonephritis lesions on the injection of monoclonal antibodies",

author = "Baghus, {W. M.} and Hoedemaeker, {P. J.} and J. Rozing and Bakker, {W. W.}",

year = "1986",

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journal = "Immunology Letters",

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T1 - Acute glomerulonephritis after intravenous injection of monoclonal anti-thymocyte antibodies in the rat

AU - Baghus, W. M.

AU - Hoedemaeker, P. J.

AU - Rozing, J.

AU - Bakker, W. W.

PY - 1986/3

Y1 - 1986/3

N2 - Female Wistar rats were injected with (mouse) monoclonal antibodies (Moabs) of different IgG subclasses directed to rat thymocytes or rat tumor cells. Following intravenous injection of antithymocyte Moabs, glomerular binding of mouse IgG was observed during the first 4 days along the GBM and in the mesangium. No staining for mouse IgG was detected in anti-tumor Moab injected rats. Animals injected with IgG 2a anti-thymocyte Moab developed glomerulonephritis and a massive proteinuria in contrast to rats injected with IgG 1 Moab which is non-complement fixing. The glomerulonephritis lesion consisted of microaneurysms and focal and segmental proliferation. Deposits of complement and fibrin could be detected exclusively in rats injected with IgG 2a anti-thymocyte Moab during the whole observation period of 14 days. This is the first demonstration of overt glomerulonephritis lesions on the injection of monoclonal antibodies

AB - Female Wistar rats were injected with (mouse) monoclonal antibodies (Moabs) of different IgG subclasses directed to rat thymocytes or rat tumor cells. Following intravenous injection of antithymocyte Moabs, glomerular binding of mouse IgG was observed during the first 4 days along the GBM and in the mesangium. No staining for mouse IgG was detected in anti-tumor Moab injected rats. Animals injected with IgG 2a anti-thymocyte Moab developed glomerulonephritis and a massive proteinuria in contrast to rats injected with IgG 1 Moab which is non-complement fixing. The glomerulonephritis lesion consisted of microaneurysms and focal and segmental proliferation. Deposits of complement and fibrin could be detected exclusively in rats injected with IgG 2a anti-thymocyte Moab during the whole observation period of 14 days. This is the first demonstration of overt glomerulonephritis lesions on the injection of monoclonal antibodies

U2 - 10.1016/0165-2478(86)90091-X

DO - 10.1016/0165-2478(86)90091-X

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JF - Immunology Letters

SN - 0165-2478

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