ADAPTATION OF XANTHOBACTER-AUTOTROPHICUS-GJ10 TO BROMOACETATE DUE TO ACTIVATION AND MOBILIZATION OF THE HALOACETATE DEHALOGENASE GENE BY INSERTION ELEMENT-IS1247

J VANDERPLOEG, G VANHALL, DB JANSSEN

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    Abstract

    Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ10M50, a DNA fragment (designated IS1247) had copied itself from a position on the chromosome that was not linked to the dhlB region to a site immediately upstream of dhlB, resulting in a 1,672-bp insertion. IS1247 was found to encode an open reading frame corresponding to 464 amino acids which showed similarity to putative transposases from two other insertion elements. In most of the ether MBA-resistant mutants of GJ10, IS1247 was also present in one more copy than in the wild type, which had two copies located within 20 kb. After insertion to a site proximal to dhlB, IS1247 was able to transpose itself together with the dhlB gene to a plasmid, without the requirement of a second insertion element being present downstream of dhlB. The results show that IS1247 causes bromoacetate resistance by overexpression and mobilization of the haloacid dehalogenase gene, which mimics steps during the evolution of a catabolic transposon and plasmid during adaptation to a toxic growth substrate.

    Original languageEnglish
    Pages (from-to)1348-1356
    Number of pages9
    JournalJournal of Bacteriology
    Volume177
    Issue number5
    Publication statusPublished - Mar-1995

    Keywords

    • PSEUDOMONAS-PUTIDA PP3
    • ALIPHATIC-COMPOUNDS
    • SEQUENCE ANALYSIS
    • DNA-SEQUENCE
    • MORAXELLA SP
    • DEGRADATION
    • PLASMID
    • CLONING
    • STRAIN
    • 1,2-DICHLOROETHANE

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