Adenosine involvement on bronchial reactivity modulation by diesel exhaust

Elena Cojocaru, Irina Luciana Dumitriu, B Gurzu, Ioana Margineanu, Maria Dinca, M Costuleanu, Simona Mihaela Slătineanu, Brigitte Scutaru, Gh Petrescu

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    UNLABELLED: In recent decades, epidemiologic investigations have suggested a strong relationship between air pollution and an increase in the prevalence of allergic rhinitis and asthma.

    AIM: To investigate the possible involvement of adenosine (AD) in bronchomotor effects of diesel exhaust (DE).

    MATERIAL AND METHOD: Isolated bronchi from ovalbumin (OVA) sensitized rats were challenged in presence or absence of diesel exhaust extract (DEE). AD was delivered on organ bath before or after DEE, at concentrations did not produce significantly contractile effects.

    RESULTS: AD (0.1 microM) pre-treatment increased bronchomotor effects of DEE: amplified the bronchoconstrictor effects of OVA with more than 35% and decreased Emax of terbutaline induced bronchorelaxation of acetylcholine (Ach) preconstricted bronchial rings (up to 20%), but did not significantly modify ACh-induced contractions. OVA-induced contractions, ACh-induced contractions and terbutaline-induced relaxations have not been significantly modified as compare with DEE alone. On the other hand, DEE amplified AD (cumulative doses) contractile effects.

    CONCLUSION: These results confirmed our initial hypothesis that AD could partial mediate or at least, modulate DEE effects on airway reactivity.

    Original languageEnglish
    Pages (from-to)180-4
    Number of pages5
    JournalRevista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi
    Volume113
    Issue number1
    Publication statusPublished - 1-Jan-2009

    Keywords

    • Acetylcholine
    • Adenosine
    • Air Pollutants
    • Algorithms
    • Animals
    • Bronchi
    • Bronchial Hyperreactivity
    • Bronchodilator Agents
    • In Vitro Techniques
    • Male
    • Ovalbumin
    • Rats
    • Rats, Wistar
    • Terbutaline
    • Vasodilator Agents
    • Vehicle Emissions
    • Journal Article

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