ADHESION OF 3 LACTOBACILLUS STRAINS TO HUMAN URINARY AND INTESTINAL EPITHELIAL-CELLS

G REID*, AL SERVIN, AW BRUCE, HJ BUSSCHER

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

Three strains of urogenital lactobacilli were found to adhere in phosphate buffered saline to human uroepithelial cells in vitro according to thermodynamic principles, and to adhere in culture medium to intestinal cells with no such correlation. The most hydrophilic strain (water contact angle 54-degrees) L. casei RC-17 was the most adherent to uroepithelial (118 bacteria per cell) and intestinal cells (165 bacteria per cell). A direct correlation was found between bacterial hydrophilicity and adhesion to uroepithelial cells for the three strains tested. An extracellular adhesin, which appeared to be proteinaceous, and a trypsin-insensitive cell wall adhesin were identified. It was evident that high levels of bacterial retention could be reproduced in vitro, perhaps indicating the potential colonizing capacity of these strains in vivo.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalMicrobios
Volume75
Issue number302
Publication statusPublished - 1993

Keywords

  • PROTEIN-MEDIATED ADHESION
  • HUMAN UROEPITHELIAL CELLS
  • ESCHERICHIA-COLI
  • COMPETITIVE-EXCLUSION
  • BACTERIAL ADHERENCE
  • HUMAN ENTEROCYTE
  • CACO-2 CELLS
  • LINE CACO-2
  • UROPATHOGENS
  • CULTURE

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