ADHESION OF MUTANS STREPTOCOCCI TO GLASS WITH AND WITHOUT A SALIVARY COATING AS STUDIED IN A PARALLEL-PLATE FLOW CHAMBER

HJ BUSSCHER*, GI DOORNBUSCH, HC VANDERMEI

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

45 Citations (Scopus)

Abstract

Deposition and adhesion to glass with and without a salivary coating in a parallel-plate flow chamber were studied with four strains of mutans streptococci. Stationary-state adhesion of the strains to uncoated glass ranged from 0.3 x 10(6) cm-2 (Streptococcus rattus HG218) to 12.7 x 10(6) cm-2 (Streptococcus sobrinus HG1025) and generally decreased after saliva coating of the glass. The poor adhesion found for S. rattus HG218 to both uncoated and saliva-coated glass could be due to its relatively high negative surface-charge. Deposition efficiencies of all strains were greater-than-or-equal-to 1 for uncoated glass and decreased greatly after saliva coating of the glass. Possibly, adhesion to a saliva coating is less efficient and more time-consuming than that to uncoated glass, because stereochemical groups in the pellicle and on the cell surfaces may have to re-arrange before an effective interaction can occur. Desorption rates, measured 1000 s and 5000 s after the start of an experiment, decreased by a factor of ten upon a five-fold increase in contact time, indicative of a two-phase adhesion process. Of the four strains studied, only Streptococcus cricetus HG737 showed a minor positive cooperativity on saliva-coated glass, possibly mediated by surface appendages observed by transmission electron microscopy on negatively-stained cells. Retention of adhering bacteria was strain-dependent on uncoated glass, but was identical for all strains on saliva-coated glass, which suggests that the structure and composition of the pellicle may be more important with respect to the retention of adhering cells than the cell-surface properties themselves.

Original languageEnglish
Pages (from-to)491-500
Number of pages10
JournalJournal of Dental Research
Volume71
Issue number3
Publication statusPublished - Mar-1992

Keywords

  • SURFACE FREE-ENERGIES
  • SOLID SUBSTRATA
  • ORAL STREPTOCOCCI
  • CELL-SURFACES
  • MICROORGANISMS
  • PARTICLES
  • SANGUIS
  • DEPOSITION
  • ADHERENCE
  • KINETICS

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