Adsorption of human immunoglobulin to implantable alginate-poly-L-lysine microcapsules: Effect of microcapsule composition

Susan K. Tam*, Bart J. de Haan, Marijke M. Faas, Jean-Pierre Halle, L'Hocine Yahia, Paul de Vos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)

Abstract

Alginate-poly-L-lysine-alginate (APA) microcapsules continue to be the most widely Studied device for the immuno-protection of transplanted therapeutic cells. Producing APA microcapsules having a reproducible and high level of biocompatibility requires an understanding of the mechanisms of the immune response towards the implants. Here, we investigate the adsorption of immunoglobulins (IgG, IgM, and IgA) onto the surface of APA microcapsules in vitro after their exposure to human serum and peritoneal fluid. Immunoglobulins (Ig) are considered to be opsonizing proteins, thus they tend to mediate inflammation when adsorbed to foreign surfaces. Ig adsorption was monitored using direct immunofluorescence. The amount of Ig adsorbed to the microcapsule surface was not significantly influenced by the guluronic acid content nor the purity level of the alginate, although microcapsules of intermediate-G purified alginate corresponded with the lowest adsorption levels. Ig adsorption was negligible when the poly-L-lysine membrane was omitted, suggesting that positive charges at the microcapsule Surface are responsible for binding Ig. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 609-615, 2009

Original languageEnglish
Pages (from-to)609-615
Number of pages7
JournalJournal of Biomedical Materials Research. Part A
Volume89A
Issue number3
DOIs
Publication statusPublished - 1-Jun-2009

Keywords

  • protein adsorption
  • immunofluorescence
  • biocompatibility
  • alginate
  • microcapsules
  • PANCREATIC-ISLETS
  • PLL CAPSULES
  • POLYLYSINE MICROCAPSULES
  • PROTEIN ADSORPTION
  • PERITONEAL-FLUID
  • MANNURONIC ACID
  • HOST-REACTION
  • IN-VITRO
  • BIOCOMPATIBILITY
  • ACTIVATION

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