Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows

Frank Klont*, Marcel Kwiatkowski, Alen Faiz, Thea van den Bosch, Simon D Pouwels, Frank J Dekker, Nick H T Ten Hacken, Péter Horvatovich, Rainer Bischoff

*Corresponding author for this work

Research output: Contribution to journalLetterAcademicpeer-review

1 Citation (Scopus)
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Abstract

Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of a captured protein from the sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g., immunoassays) but hardly ever prior to liquid chromatography-mass spectrometry (LC-MS-)-based detection. Here, we describe the use of adsorptive microtiter plates for protein enrichment prior to LC-MS detection, and we discuss opportunities and challenges of corresponding workflows, based on examples of targeted (i.e., soluble receptor for advanced glycation end-products (sRAGE) in human serum) and discovery-based workflows (i.e., transcription factor p65 (NF-κB) in lysed murine RAW 264.7 macrophages and peptidyl-prolyl cis-trans isomerase FKBP5 (FKBP5) in lysed human A549 alveolar basal epithelial cells). Thereby, we aim to highlight the potential usefulness of adsorptive microtiter plates in affinity purification workflows prior to LC-MS detection, which could increase their usage in mass spectrometry-based protein research.

Original languageEnglish
Pages (from-to)5218–5221
Number of pages4
JournalJournal of Proteome Research
Volume20
Issue number11
Early online date2021
DOIs
Publication statusPublished - 5-Nov-2021

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