@inbook{a59a2f0a312c4fcd93e6e6be7f5454ae,
title = "Affinity-Based Interactome Analysis of Endogenous LINE-1 Macromolecules",
abstract = "During their proliferation and the host{\textquoteright}s concomitant attempts to suppress it, LINE-1 (L1) retrotransposons give rise to a collection of heterogeneous ribonucleoproteins (RNPs); their protein and RNA compositions remain poorly defined. The constituents of L1-associated macromolecules can differ depending on numerous factors, including, for example, position within the L1 life cycle, whether the macromolecule is productive or under suppression, and the cell type within which the proliferation is occurring. This chapter describes techniques that aid the capture and characterization of protein and RNA components of L1 macromolecules from tissues that natively express them. The protocols described have been applied to embryonal carcinoma cell lines that are popular model systems for L1 molecular biology (e.g., N2102Ep, NTERA-2, and PA-1 cells), as well as colorectal cancer tissues. N2102Ep cells are given as the use case for this chapter; the protocols should be applicable to essentially any tissue exhibiting endogenous L1 expression with minor modifications.",
keywords = "Affinity capture, Interactomics, Protein complexes, Retrotransposon, Ribonucleoprotein",
author = "\{Di Stefano\}, \{Luciano H.\} and Saba, \{Leila J.\} and Mehrnoosh Oghbaie and Hua Jiang and Wilson McKerrow and Maria Benitez-Guijarro and Taylor, \{Martin S.\} and John LaCava",
note = "Funding Information: This work was supported in-part by National Institutes of Health grants R01GM126170 and P41GM109824. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2883-6\_12",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "215--256",
booktitle = "Methods in Molecular Biology",
}