Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

Pol Besenius*, Peter A. G. Cormack, R. Frederick Ludlow, Sijbren Otto, David C. Sherrington

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)
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Abstract

We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.

Original languageEnglish
Pages (from-to)2414-2418
Number of pages5
JournalOrganic & Biomolecular Chemistry
Volume8
Issue number10
DOIs
Publication statusPublished - 2010

Keywords

  • DRIVE CHEMICAL EVOLUTION
  • MOLECULAR RECOGNITION
  • MACROCYCLIC DISULFIDES
  • SIMULTANEOUS SELECTION
  • SYNTHETIC RECEPTORS
  • COVALENT CHEMISTRY
  • GUEST BINDING
  • WATER
  • DISCOVERY
  • TEMPLATES

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