Allele and haplotype frequencies for HLA-DQ in Iranian celiac disease patients

Mohammad Rostami-Nejad, Jihane Romanos, Kamran Rostami, Azita Ganji, Mohammad Javad Ehsani-Ardakani*, Ali-Reza Bakhshipour, Homayoun Zojaji, Seyed Reza Mohebbi, Mohammad-Reza Zali, Cisca Wijmenga

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)
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Abstract

AIM: To assess the distribution of human leukocyte antigen (HLA)-DQ2 and -DQ8 in Iranian celiac disease (CD) patients and compare them to healthy Iranian controls.

METHODS: To predict the HLA-DQA1 and -DQB1 genes, we used six previously reported HLA-tagging single nucleotide polymorphism to determine HLA genotypes in 59 Iranian patients with 'biopsy-confirmed' CD and in 151 healthy Iranian individuals. To test the transferability of the method, 50 cases and controls were also typed using a commercial kit that identifies individual carriers of DQ2, DQ7 and DQ8 alleles.

RESULTS: In this pilot study 97% of CD cases (n = 57) and 58% of controls (n = 87) were carriers of HLA-DQ2 and/or HLA-DQ8 heterodimers, either in the homozygous or heterozygous state. The HLA-DQ pattern of these 57 CD patients: heterozygous DQ2.2 (n = 14) and homozygous DQ2.2 (n = 1), heterozygous DQ2.5 (n = 33) and homozygous DQ2.5 (n = 8), heterozygous DQ8 (n = 13) and homozygous DQ8 (n = 2). Two CD patients were negative for both DQ2 and DQ8 (3%).

CONCLUSION: The prevalence of DQ8 in our CD population was higher than that reported in other populations (25.4%). As reported in other populations, our results underline the primary importance of HLA-DQ alleles in the Iranian population's susceptibility to CD. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

Original languageEnglish
Pages (from-to)6302-6308
Number of pages7
JournalWorld Journal of Gastroenterology
Volume20
Issue number20
DOIs
Publication statusPublished - 28-May-2014

Keywords

  • Human leukocyte antigen typing
  • Validation
  • Susceptibility
  • Celiac disease
  • Iran
  • RISK
  • PATHOGENESIS
  • ASSOCIATION
  • PREVALENCE
  • DIAGNOSIS

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