An early-life diet containing large phospholipid-coated lipid globules programs later-life postabsorptive lipid trafficking in high-fat diet but not in low-fat dietfed mice

Onne A H O Ronda, Bert J M van de Heijning, Ingrid A Martini, Martijn Koehorst, Rick Havinga, Angelika Jurdzinski, Vincent W Bloks, Theo H van Dijk, Eline M van der Beek, Folkert Kuipers, Henkjan J Verkade*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Feeding mice in early-life a diet containing an experimental infant milk formula (Nuturis®; eIMF), with a lipid structure similar to human milk, transiently lowered body weight and fat mass gain upon Western-style diet later in life, when compared to mice fed diets based on control IMF (cIMF). We tested the hypothesis that early-life eIMF feeding alters the absorption or the postabsorptive trafficking of dietary lipids in later-life. Male C57BL/6JOlaHsd mice were fed eIMF/cIMF from postnatal day 16-42, followed by low- (LFD, AIN-93G, 7wt% fat) or high-fat diet (HFD, D12451, 24wt% fat) until day 63-70. Lipid absorption rate and tissue concentrations were determined after intragastric administration of stable isotope (deuterium or 13C) labelled lipids in separate groups. Lipid enrichments in plasma and tissues were analysed using gas chromatography-mass spectrometry. The rate of triolein absorption was similar between eIMF and cIMF fed LFD: 3.2 SD 1.8 and 3.9 SD 2.1 and HFD: 2.6 SD 1.7 and 3.8 SD 3.0 Postabsorptive lipid trafficking, i.e., concentrations of absorbed lipids in tissues, was similar in the eIMF and cIMF groups after LFD. Tissue levels of absorbed triglycerides after HFD-feeding were lower in heart (-42%) and liver (-46%), and higher in muscle (+81%, all p<0.05) in eIMF-fed mice. In conclusion, early-life IMF diet affected postabsorptive trafficking of absorbed lipids after HFD, but not LFD. Changes in postabsorptive lipid trafficking could underlie the observed lower body weight and body fat accumulation in later life upon a persistent long-term obesogenic challenge.

Original languageEnglish
Article number0007114520002421
Pages (from-to)961-971
Number of pages11
JournalBritish Journal of Nutrition
Issue number9
Early online date3-Jul-2020
Publication statusPublished - 14-May-2021


  • Metabolic programming
  • Infant nutrition
  • Dietary lipids
  • Lipid structure
  • Milk fat globule membrane
  • Obesity prevention

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